Villamor Eduardo, Degraeuwe Pieter L J, De Mey Jo G R, Blanco Carlos E
Department of Pediatrics, University Hospital Maastricht, Research Institute Growth and Development (GROW), University of Maastricht, P. Debyelaan 25, PO Box 5800, NL-6202 AZ Maastricht, The Netherlands.
Biol Neonate. 2003;84(2):172-8. doi: 10.1159/000071953.
This study aimed to evaluate the effect of tidal liquid ventilation (TLV) and liquid assisted high frequency oscillatory ventilation (LA-HFOV) on the contractile and relaxing properties of fetal ovine pulmonary arteries. 0.85 term lambs were subjected, after surfactant instillation, to 5 h of TLV or LA-HFOV (5 ml.kg(-1) perfluorocarbon liquid). After euthanasia of the animals, intrapulmonary arteries (fourth branch) were dissected and mounted in a myograph for isometric tension recording. Arteries from unventilated age-matched fetuses were also studied. The contractions induced by K(+), and the thromboxane A(2) mimetic U46619 were not significantly different in the TLV and the LA-HFOV groups. Acetylcholine-induced relaxation was absent after TLV and LA-HFOV but present in unventilated animals. Sodium nitroprusside-induced relaxation was also similar after TLV and LA-HFOV, but reduced when the two groups were compared with unventilated fetuses. We conclude that after TLV and LA-HFOV the pulmonary arterial responses to receptor- and non receptor-mediated contraction and to endothelium-dependent and -independent relaxation were similar.
本研究旨在评估潮式液体通气（TLV）和液体辅助高频振荡通气（LA-HFOV）对胎羊肺动脉收缩和舒张特性的影响。0.85足月羔羊在注入表面活性剂后，接受5小时的TLV或LA-HFOV（5毫升·千克⁻¹全氟化碳液体）。动物安乐死后，解剖肺内动脉（第四分支）并安装在肌动描记器上进行等长张力记录。还研究了未通气的年龄匹配胎儿的动脉。在TLV组和LA-HFOV组中，钾离子（K⁺）和血栓素A₂模拟物U46619诱导的收缩无显著差异。TLV和LA-HFOV后乙酰胆碱诱导的舒张消失，但在未通气的动物中存在。硝普钠诱导的舒张在TLV和LA-HFOV后也相似，但与未通气的胎儿相比，两组均降低。我们得出结论，在TLV和LA-HFOV后，肺动脉对受体介导和非受体介导的收缩以及对内皮依赖性和非依赖性舒张的反应相似。
