Accorsi Roberto, Morowitz Michael J, Charron Martin, Maris John M
Division of Nuclear Medicine and Department of Radiology, The Children's Hospital of Philadelphia, 34th and Civic Center Blvd., PA 19104, Philadelphia, USA.
Pediatr Radiol. 2003 Oct;33(10):688-92. doi: 10.1007/s00247-003-1006-6. Epub 2003 Aug 8.
To evaluate (131)I-MIBG scintigraphic localization of xenotransplanted and spontaneously arising neuroblastomas in murine models of high-risk neuroblastoma.
Neuroblastoma xenografts were created by inoculation of human neuroblastoma cell suspensions into the subcutaneous flanks of athymic nude mice. In addition, spontaneous paraspinal neuroblastomas were detected by direct palpation in MYCN transgenic mice. After measured tumor volumes exceeded 200 mm(3), each mouse received an intraperitoneal injection of 18 muCi/g (131)I-metaiodobenzylguanidine ((131)I-MIBG). Pinhole scintigraphy was performed to evaluate the MIBG biodistribution and to attempt to visualize the tumors. Each mouse was imaged on a gamma camera equipped with a 3-mm pinhole on one head and an HEGP collimator on the other.
Images demonstrated absorption of radiolabeled MIBG and visualization of tumors. Analysis of the images allowed for quantification of relative MIBG uptake and for determination of linear and area measurements of the tumors.
High-energy pinhole imaging effectively demonstrates uptake of radiolabeled MIBG by human neuroblastoma tumors in murine laboratory models. This technique allows for in vivo assessment of tumor burden. In the future, we plan to use this method to evaluate sensitivity for detecting metastatic spread as well as investigating the therapeutic efficacy of high-dose (131)I-MIBG in combination with radiosensitizing agents.
评估¹³¹I-间碘苄胍(¹³¹I-MIBG)闪烁显像对高危神经母细胞瘤小鼠模型中异种移植及自发产生的神经母细胞瘤的定位情况。
通过将人神经母细胞瘤细胞悬液接种到无胸腺裸鼠的侧腹皮下创建神经母细胞瘤异种移植模型。此外,通过直接触诊在MYCN转基因小鼠中检测自发的脊柱旁神经母细胞瘤。在测量的肿瘤体积超过200 mm³后,每只小鼠腹腔注射18 μCi/g的¹³¹I-间碘苄胍(¹³¹I-MIBG)。进行针孔闪烁显像以评估MIBG的生物分布并尝试使肿瘤显影。每只小鼠在一台γ相机上成像,该相机一头配备3毫米针孔,另一头配备高能通用准直器(HEGP)。
图像显示了放射性标记的MIBG的摄取及肿瘤的显影。对图像的分析允许对相对MIBG摄取进行定量,并确定肿瘤的线性和面积测量值。
高能针孔成像有效地显示了小鼠实验模型中人类神经母细胞瘤肿瘤对放射性标记的MIBG的摄取。该技术允许对肿瘤负荷进行体内评估。未来,我们计划使用这种方法评估检测转移扩散的敏感性,以及研究高剂量¹³¹I-MIBG与放射增敏剂联合使用的治疗效果。