[Leukocytolysis and phagocytosis under hyperosmotic conditions].

PAN and leukocytolysis were measured in dog blood placed in hypo- and hypertonic solutions of glycerin and glucose (1:10). It was found that leukocytolytic activity of the substances depends on equimolar concentrations of nonelectrolytes. It is more pronounced in hypo- than in hypertonic conditions. Glycerin is 3-5-times more active than glucose. In concentrations 2.0 M for glycerin and 0.5 M for glucose leukocytolysis is less active. Mononuclears are more resistant than neutrophils in strong solutions. PAN increased by 11-16% in hypotonic solutions. Strong glycerin solutions reduce PAN less than glucose which stimulates the digestive activity. The results can be of use in specific leukolysis tests to diagnose drug allergy.