[从抗生素相关性腹泻（AAD）分离出的艰难梭菌菌株中检测对克林霉素具有高水平耐药性的ermB基因（MLS型耐药）]
[Detection of ermB gene responsible for high level resistance to clindamycin (MLS type resistance) among Clostridium difficile strains isolated from antibiotic associated diarrhea (AAD)].
作者信息
Pituch Hanna, Obuch-Woszczatyński Piotr, Meisel-Mikołajczyk Felicja, Łuczak Miroslaw
机构信息
Katedra i Zakład Mikrobiologii Lekarskiej AM w Warszawie.
出版信息
Med Dosw Mikrobiol. 2003;55(1):61-6.
In 68 C. difficile strains isolated from feacal samples of patients with antibiotic associated diarrhoea (AAD) investigated presence of ermB gene transferable of high level resistance to clindamycin. The primers set 2980/2981 used for identification of ermB gene amplified a 688 bp segment. We used the Etest to assess all strains for susceptibility to clindamycin. This study demonstrates that 57% of strains isolated from faecal samples of patients with AAD were highly resistant to clindamycin (minimal inhibitory concentration (MIC) of clindamycin, 256 mg/L) and possessed the ermB gene.
在从患有抗生素相关性腹泻（AAD）患者的粪便样本中分离出的68株艰难梭菌菌株中，研究了可转移的ermB基因对克林霉素的高水平耐药性。用于鉴定ermB基因的引物对2980/2981扩增出一个688 bp的片段。我们使用Etest评估所有菌株对克林霉素的敏感性。本研究表明，从AAD患者粪便样本中分离出的57%的菌株对克林霉素高度耐药（克林霉素的最低抑菌浓度（MIC）为256 mg/L）且拥有ermB基因。
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