Zareba Tomasz, Bocian Ewa, Tyski Stefan
Zakład Antybiotyków i Mikrobiologii Narodowego Instytutu Zdrowia Publicznego w Warszawie.
Med Dosw Mikrobiol. 2003;55(1):97-104.
Standards PN-EN 1040 and EN 12054 describe test methods and minimum requirements for bactericidal activities of antiseptics. However, standard procedures are time consuming and require 48 hours of incubation. New alternative technique based on impedimetric procedure provides possibilities to reduce this time to several hours. The Bactometer (BioMerieux, Vitek System, USA) is a fully automated impedance technology system used to microbial quantitation of products. Impedance measures microbial activity by electrical methods. The aim of the study was to adaptate the impedimetric method utilising Bactometer--system to microbiological activity control of chemical antiseptics. Eight different products were utilised throughout the study. The samples for classical and alternative method were prepared in the same way as described in standards. The method of choice was dilution-neutralization method. All procedures conducted in Bactometer were verified by plate count method. The high correlation was observed between results obtained by normative methods and impedimetric measurement. All tested products meet requirements. The procedure utilising the Bactometer, provides a rapid and accurate system for the determination of bacterial content. The results of validation carried out during this study indicate, possibility to use impedimetric method alternatively to traditional methods.
PN-EN 1040标准和EN 12054标准描述了防腐剂杀菌活性的测试方法和最低要求。然而，标准程序耗时且需要48小时的培养时间。基于阻抗测量程序的新替代技术有可能将这段时间缩短至数小时。Bactometer（美国生物梅里埃公司，Vitek系统）是一种用于产品微生物定量的全自动阻抗技术系统。阻抗通过电学方法测量微生物活性。本研究的目的是使利用Bactometer系统的阻抗测量方法适用于化学防腐剂的微生物活性控制。整个研究使用了八种不同的产品。经典方法和替代方法的样品按照标准中所述的相同方式制备。选择的方法是稀释中和法。在Bactometer中进行的所有程序均通过平板计数法进行验证。在标准方法获得的结果与阻抗测量结果之间观察到高度相关性。所有测试产品均符合要求。使用Bactometer的程序为细菌含量的测定提供了一种快速且准确的系统。在本研究期间进行的验证结果表明，可以用阻抗测量方法替代传统方法。
