Dorn S, Natale D, Mattiacci L, Hern A, Pasqualini E, Dorn S
Institute of Plant Sciences Applied Entomology, Swiss Federal Institute of Technology (ETH) Clausiusstrasse 25/NW Zurich CH-8092 Switzerland.
Bull Entomol Res. 2003 Aug;93(4):335-42. doi: 10.1079/ber2003250.
Peach shoot volatiles were attractive to mated female oriental fruit moth, Cydia molesta (Busck), in a dual choice arena. No preference was observed between leaf odours from the principle host plant, peach, and the secondary host plant, apple. Twenty-two compounds were identified in headspace volatiles of peach shoots using gas chromatography-mass spectrometry. Green leaf volatiles accounted for more than 50% of the total emitted volatiles. A bioassay-assisted fractionation using different sorbent polymers indicated an attractant effect of compounds with a chain length of 6-8 carbon atoms. The major compounds of this fraction were tested either singly or in combinations for behavioural response of females. Significant bioactivity was found for a three-component mixture of (Z)-3-hexen-1-yl acetate, (Z)-3-hexen-1-ol and benzaldehyde in a 4:1:1 ratio. This synthetic mixture elicited a similar attractant effect as the full natural blend from peach shoots as well as the bioactive fraction.
在双选试验场中，桃梢挥发物对已交配的雌性梨小食心虫（Cydia molesta (Busck)）具有吸引力。在主要寄主植物桃和次要寄主植物苹果的叶片气味之间未观察到偏好。利用气相色谱 - 质谱联用技术在桃梢顶空挥发物中鉴定出22种化合物。绿叶挥发物占总挥发物排放量的50%以上。使用不同吸附剂聚合物进行的生物测定辅助分级分离表明，碳链长度为6 - 8个碳原子的化合物具有引诱作用。对该级分的主要化合物单独或组合测试其对雌虫的行为反应。发现(Z)-3-己烯-1-基乙酸酯、(Z)-3-己烯-1-醇和苯甲醛以4:1:1比例组成的三元混合物具有显著的生物活性。这种合成混合物产生的引诱效果与桃梢的全天然混合物以及生物活性级分相似。
