Jo C, Yook H S, Lee M S, Kim J H, Song H P, Kwon J S, Byun M W
Team for Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute, Yuseong, 305-600 Daejeon, South Korea.
Food Chem Toxicol. 2003 Oct;41(10):1329-36. doi: 10.1016/s0278-6915(03)00125-x.
Gossypol dissolved in methanol (0.25 and 0.5 mg/ml) was gamma-irradiated at 0, 5, 10, and 20 kGy. The gossypol content was significantly reduced by irradiation in a dose-dependent manner. Ames test performed with nonirradiated and irradiated gossypol solutions was negative at the level of 0.1, 0.5, 1, 10, 50, and 100 microg gossypol/plate. At the gossypol concentration of 2.5 microg/ml, the blastocysts formation rate of mice embryo were decreased by a factor of two as compared with the control, but irradiation at 20 kGy increased the blastocysts formation, resulting in no difference from the control. Addition of gossypol to oil emulsion system or oil showed a prooxidative effect of lipids at the beginning stage by increasing 2-thiobarbituric acid reactive substances (TBARS) and peroxide value but irradiation of the gossypol solution decreased the oxidative changes significantly as compared with the nonirradiated one. Results indicated that irradiation decreased the gossypol concentration dissolved in methanol, resulting in a reduction of embryotoxicity in mice.
将溶解于甲醇（0.25和0.5毫克/毫升）中的棉酚分别以0、5、10和20千戈瑞的剂量进行γ射线辐照。辐照使棉酚含量以剂量依赖的方式显著降低。用未辐照和辐照过的棉酚溶液进行的艾姆斯试验在棉酚含量为0.1、0.5、1、10、50和100微克/平板时呈阴性。在棉酚浓度为2.5微克/毫升时，与对照组相比，小鼠胚胎的胚泡形成率降低了一半，但20千戈瑞的辐照增加了胚泡形成，与对照组无差异。在油乳液体系或油中添加棉酚，在开始阶段通过增加硫代巴比妥酸反应性物质（TBARS）和过氧化值显示出脂质的促氧化作用，但与未辐照的棉酚溶液相比，辐照过的棉酚溶液显著降低了氧化变化。结果表明，辐照降低了溶解于甲醇中的棉酚浓度，从而降低了对小鼠的胚胎毒性。
