Park S-U, Chae Y-A, Facchini P J
Department of Biological Sciences, University of Calgary, T2N 1N4, Calgary, Alberta, Canada.
Plant Cell Rep. 2003 Aug;21(12):1194-8. doi: 10.1007/s00299-003-0639-0. Epub 2003 May 13.
Scrophularia buergeriana Miq. (figwort) contains a diverse group of bioactive natural products and is used to treat a variety of ailments, including fever, constipation, neuritis, and laryngitis. A transformation protocol was established for S. buergeriana using Agrobacterium tumefaciens. Kanamycin-resistant plants were regenerated from leaf explants co-cultivated with A. tumefaciens strain GV3101. The shoot regeneration medium was supplemented with 2 mg l(-1) 6-benzylaminopurine and 70 mg l(-1) putrescine to improve the efficiency of organogenesis. Detection of the neomycin phosphotransferase gene, the presence of high levels of beta-glucuronidase (GUS) transcripts and enzyme activity, and the histochemical localization of GUS confirmed the genetic transformation of S. buergeriana. This work demonstrates the potential of using A. tumefaciens to efficiently transfer foreign genes into a commercially and culturally important Oriental medicinal plant.
玄参含有多种具有生物活性的天然产物，可用于治疗多种疾病，包括发烧、便秘、神经炎和喉炎。利用根癌农杆菌建立了玄参的转化体系。用根癌农杆菌菌株GV3101共培养叶片外植体，再生出了卡那霉素抗性植株。在芽再生培养基中添加2 mg l(-1) 6-苄基腺嘌呤和70 mg l(-1) 腐胺，以提高器官发生效率。新霉素磷酸转移酶基因的检测、高水平β-葡萄糖醛酸酶（GUS）转录本和酶活性的存在以及GUS的组织化学定位证实了玄参的遗传转化。这项工作证明了利用根癌农杆菌将外源基因有效导入一种具有商业和文化重要性的东方药用植物的潜力。
