Baĭdun L V, Kashpor S A, Parpara A A, Pliasunova S A, Piatnitskiĭ A M, Smetanina N S, Sokolinskiĭ B Z
Klin Lab Diagn. 2003 Jun(6):39-42.
Measurements of the form and size of erythrocytes are needed in the diagnosis of a number of diseases. However, such measurements, if made manually, are a labor-consuming and often inaccurate method, which ensures the determination of a very limited number of parameters. Hardware/software unit MEKOS-C1 enables an automated examination of blood smear, thus speeding up significantly the analysis and ensuring a more complete and accurate information. The possibilities of unit MEKOS-C1 were evaluated for the diagnosis of ovalocytosis. Blood smears of 19 patients from the Russian Pediatric Clinical Hospital, including 8 patients with inherited ovalocytosis, 1 patient with spherocytic ovalocytosis and 10 patients without the disease, were made use of Erythrocytes were isolated in the images of preparations and the contour of each cell was approximated by ellipse. The ratio between ellipse semi-axes served as a measure of erythrocytes' ovality. The mean ratio of semi-axes (RS) and the index of ovalocytosis (IO), i.e. a ratio of the mean maximum diameter to the mean minimal diameter, were calculated for each smear. Manual IO measurements were made in all preparations as a control. Since an additional error can enter the result because of the irregular smear nature and impossibility to standardize completely the technology of smear preparation, the data, obtained from two different smear parts and from parallel preparations, were compared. The reliability, stability and good reproducibility of the automated measurement results were demonstrated. The mean erythrocytes' RS correlated well with IO, obtained manually, and did not virtually differ from RS, measured in the automated manner. The mean RS value of erythrocytes, obtained from patients with inherited ovalocytosis, significantly differed from the control values, which is indicative of a high information density of the discussed parameter. Therefore, RS, when measured automatically, is a reliable and convenient characteristic of erythrocytes' ovality under the conditions of using the ordinary technique of smear preparation.
在多种疾病的诊断中，需要对红细胞的形态和大小进行测量。然而，此类测量若采用手工方式，既耗时又往往不准确，只能确定非常有限的参数。硬件/软件单元MEKOS-C1能够对血涂片进行自动检查，从而显著加快分析速度，并确保获得更完整、准确的信息。对MEKOS-C1单元在椭圆形红细胞增多症诊断中的应用可能性进行了评估。利用了俄罗斯儿科临床医院19名患者的血涂片，其中包括8例遗传性椭圆形红细胞增多症患者、1例球形椭圆形红细胞增多症患者和10例无该疾病的患者。在制备物图像中分离出红细胞，并用椭圆拟合每个细胞的轮廓。椭圆半轴之比用作红细胞椭圆形的度量。计算每张涂片的半轴平均比（RS）和椭圆形红细胞增多症指数（IO），即平均最大直径与平均最小直径之比。对所有制备物进行手工IO测量作为对照。由于涂片性质不规则以及无法完全标准化涂片制备技术，可能会给结果带来额外误差，因此对从两个不同涂片部位以及平行制备物获得的数据进行了比较。结果表明自动测量结果具有可靠性、稳定性和良好的可重复性。红细胞的平均RS与手工获得的IO相关性良好，与自动测量的RS几乎没有差异。遗传性椭圆形红细胞增多症患者红细胞的平均RS值与对照值显著不同，这表明所讨论参数具有较高的信息密度。因此，在使用普通涂片制备技术的条件下，自动测量的RS是红细胞椭圆形的可靠且便捷的特征。
