Tang Se, Li Yunxing, Wang Meijing, Yang Chongli
Department of Pediatrics, West China Second Hospital Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Jul;34(3):398-400.
To investigate the expansion of the CD34+ cells of umbilical cord blood in vitro culture comprising different cytokines and the potential hematopoiesis function of the expanded CD34+ cells.
Expanding the purified CD34+ cells from the fresh umbilical cord blood for 6 days in vitro with the use of different combination of FL, SCF, TPO, IL-3 and IL-6. Getting one better combination by analyses of the results from flowcytometry, LTC-IC, and progenitor clonogenic assays.
The numbers of CD34+ cells expanded by use of different combination of cytokines were 3.1-8.6 times as large as the numbers of ordinary ones, thus making 16 cases of insufficient CD34+ cells become able to satisfy the needs of the adults weighing 50 kg. No significant difference was observed between the function of the expanded cells and the original ones in hematopoiesis. Meanwhile, it was proved that TPO in combination with other cytokines could strengthen the expansion of CD34+ cells, but using TPO alone for long time culture would cause more CD34+ cells to be megalocaryocytes.
The combination of FST36 cytokines can expand the CD34+ cells of the umbilical cord blood to meet the adults' needs in laboratory's methods.
研究不同细胞因子组合体外培养脐血CD34+细胞的扩增情况及扩增后CD34+细胞的潜在造血功能。
采用FL、SCF、TPO、IL-3和IL-6的不同组合，对新鲜脐血中纯化的CD34+细胞进行体外培养6天。通过流式细胞术、长期培养起始细胞（LTC-IC）和祖细胞克隆形成试验的结果分析，得出一种较好的组合。
不同细胞因子组合扩增后的CD34+细胞数量是普通CD34+细胞数量的3.1 - 8.6倍，使16例CD34+细胞不足的情况能够满足50 kg成年人的需求。扩增后细胞与原细胞在造血功能上无显著差异。同时证明，TPO与其他细胞因子联合可增强CD34+细胞的扩增，但长时间单独使用TPO培养会使更多的CD34+细胞分化为巨核细胞。
FST36细胞因子组合可在实验室方法下扩增脐血CD34+细胞以满足成人需求。
