Zhang Hongying, Liu Weiping, Wang Xiaojie, Li Gandi, Guo Jia, Li Fengyuan, Liao Dianying
Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Jul;34(3):424-6.
To evaluate the potential diagnostic role of A103 and inhibin alpha in adrenocortical adenoma and the feasibility of tissue microarray/tissue chips.
A series of adrenal gland tissue chips were prepared, including 79 cases of formalin-fixed, paraffin-embedded tissue from 3 normal adrenal glands, 66 adrenocortical adenomas, 5 cases of metastatic carcinoma and 5 pheochromocytomas. A103 and inhibin alpha were detected with Envision immunohistochemical staining methods.
Positive staining was seen in 3 of 3 (100%) adrenal cortices and in 55 of 61(90.2%) adrenocortical adenomas but in none of the pheochromocytomas or adrenal metastatic carcinomas. In the 3 of 3(100%) adrenal cortices, inhibin alpha immunoreactivity was limited to the zona reticularis and the innermost zona fasciculata. 51 of 61(83.6%) adrenocortical adenomas reacted with inhibin alpha. None of the pheochromocytomas or metastatic carcinomas reacted with inhibin alpha.
High-throughput tissue microarray/tissue chip offers a powerful strategy for rapid identification in situ. It is feasible to utilize tissue microarray for a rapid, economic and accurate screening of tissue specimens on a large scale. The combined use of A103 and inhibin alpha is valuable in distinguishing adrenocortical adenoma from pheochromocytoma and other metastatic carcinomas.
评估A103和抑制素α在肾上腺皮质腺瘤中的潜在诊断作用以及组织微阵列/组织芯片的可行性。
制备一系列肾上腺组织芯片，包括来自3个正常肾上腺、66例肾上腺皮质腺瘤、5例转移性癌和5例嗜铬细胞瘤的79例福尔马林固定、石蜡包埋组织。采用Envision免疫组织化学染色方法检测A103和抑制素α。
3个肾上腺皮质中的3个（100%）以及61个肾上腺皮质腺瘤中的55个（90.2%）呈阳性染色，但嗜铬细胞瘤或肾上腺转移性癌均未呈阳性染色。在3个肾上腺皮质中的3个（100%）中，抑制素α免疫反应仅限于网状带和最内层的束状带。61个肾上腺皮质腺瘤中的51个（83.6%）与抑制素α反应。嗜铬细胞瘤或转移性癌均未与抑制素α反应。
高通量组织微阵列/组织芯片为原位快速鉴定提供了有力策略。利用组织微阵列对组织标本进行大规模快速、经济且准确的筛查是可行的。联合使用A103和抑制素α在区分肾上腺皮质腺瘤与嗜铬细胞瘤及其他转移性癌方面具有重要价值。
