[整合素对人精子激活作用的研究]
[Study on the effect of integrin on human sperm activation].
作者信息
Xie Yimin, Yue Limin, He Yaping, Zhang Jinhu, Mao Rongqiu, Lei Song, Zheng Yu
机构信息
Department of Physiology, West China School of Basic and Forensic Medical Sciences, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Jul;34(3):459-61.
OBJECTIVE
This study was aimed to disclose whether the VLA-5 integrin on human sperm membrane takes part in the activation of human spermatozoa.
METHODS
Fluo-3/AM was coincubated with human spermatozoa. The change of intracellular calcium([Ca2+]i) was determined by flow cytometry when human sperms were exposed to Fibronectin(Fn) as the specific ligand of integrin VLA-5. Human sperm acrosome reaction(AR) rate was evaluated by triple-stain technique.
RESULTS
Fn significantly raised the AR rate (P < 0.05) and the level of [Ca2+]i (P < 0.01) in the spermatozoa of fertile men. But there was no significant rise in the level of [Ca2+]i in the spermatozoa of infertile men.
CONCLUSION
The VLA-5 integrin on human sperm membrane plays a role in the activation of sperm.
目的
本研究旨在揭示人精子膜上的VLA-5整合素是否参与人精子的激活过程。
方法
将Fluo-3/AM与人精子共同孵育。当人精子暴露于作为整合素VLA-5特异性配体的纤连蛋白（Fn）时，通过流式细胞术测定细胞内钙（[Ca2+]i）的变化。采用三重染色技术评估人精子顶体反应（AR）率。
结果
Fn显著提高了生育男性精子的AR率（P < 0.05）和[Ca2+]i水平（P < 0.01）。但不育男性精子的[Ca2+]i水平没有显著升高。
结论
人精子膜上的VLA-5整合素在精子激活中起作用。
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