Willing Alison E, Vendrame Martina, Mallery Jennifer, Cassady C Jordan, Davis Cyndy D, Sanchez-Ramos Juan, Sanberg Paul R
Center of Excellence for Aging & Brain Repair, Department of Neurosurgery, University of South Florida College of Medicine, 12901 Bruce B. Downs Blvd, Tampa, FL 33612, USA.
Cell Transplant. 2003;12(4):449-54. doi: 10.3727/000000003108746885.
Filgratism (granulocyte colony stimulating factor, G-CSF)-mobilized peripheral blood progenitor cells (PBPCs) have replaced bone marrow (BM) as a preferred source of autologous stem cells, in light of the faster hematologic recovery and lesser supportive care requirement exhibited by PBPC transplants. Other hematopoietic stem cells, like the human umbilical cord blood-derived stem cells (hUCBs), and nonhematopoietic stem cells have been shown to improve motor function in rodent models of injury and degenerative disease. In the present study we transplanted either G-CSF-mobilized PBPCs or hUCBs in rats 24 h after permanent middle cerebral artery occlusion (MCAO), and assessed their behavioral abnormalities in spontaneous activity and spontaneous motor asymmetry. In both transplanted groups of rats we observed a significant reduction of the stroke-induced hyperactivity compared with nontransplanted, stroked animals. In addition, transplantation of G-CSF PBPC and hUCB cells prevented the development of extensive motor asymmetry. Our findings raise the possibility that PBPCs could provide a novel transplantation therapy to treat stroke.
鉴于外周血祖细胞（PBPC）移植后血液学恢复更快且支持治疗需求更少，粒细胞集落刺激因子（G-CSF）动员的外周血祖细胞已取代骨髓成为自体干细胞的首选来源。其他造血干细胞，如人脐带血来源的干细胞（hUCB），以及非造血干细胞，已被证明可改善损伤和退行性疾病啮齿动物模型的运动功能。在本研究中，我们在大鼠永久性大脑中动脉闭塞（MCAO）24小时后移植G-CSF动员的PBPC或hUCB，并评估它们在自发活动和自发运动不对称方面的行为异常。与未移植、中风的动物相比，在两组移植大鼠中我们均观察到中风诱导的活动亢进显著降低。此外，G-CSF PBPC和hUCB细胞移植可防止广泛运动不对称的发展。我们的研究结果提示，PBPC有可能提供一种治疗中风的新型移植疗法。
