Koga Yosuke, Sone Nobuhito, Noguchi Shunsuke, Morii Hiroyuki
Department of Chemistry, University of Occupational and Environmental Health, Kitakyushu, Japan.
Biosci Biotechnol Biochem. 2003 Jul;67(7):1605-8. doi: 10.1271/bbb.67.1605.
sn-Glycerol-1-phosphate dehydrogenase is responsible for the formation of the sn-glycerol-1-phosphate backbone of archaeal lipids. [4-3H]NADH that had 3H at the R side was produced from [4-3H]NAD and glucose with glucose dehydrogenase (a pro-S type enzyme). The 3H of this [4-3H]NADH was transferred to dihydroxyacetonephosphate during the sn-glycerol-1-phosphate dehydrogenase reaction. On the contrary, in a similar reaction using alcohol dehydrogenase (a pro-R type enzyme), 3H was not incorporated into glycerophosphate. These results confirmed a prediction of the tertiary structure of sn-glycerol-1-phosphate dehydrogenase by homology modeling.
sn-甘油-1-磷酸脱氢酶负责古菌脂质的sn-甘油-1-磷酸主链的形成。在葡萄糖脱氢酶（一种前-S型酶）作用下，[4-³H]NAD和葡萄糖产生了在R侧带有³H的[4-³H]NADH。在sn-甘油-1-磷酸脱氢酶反应过程中，这种[4-³H]NADH的³H被转移到磷酸二羟丙酮上。相反，在使用醇脱氢酶（一种前-R型酶）的类似反应中，³H没有掺入甘油磷酸中。这些结果证实了通过同源建模对sn-甘油-1-磷酸脱氢酶三级结构的预测。
