Lee Sang Whon
Department of Preventive Medicine, School of Medicine, Kyungpook National University, Korea.
Kisaengchunghak Chapchi. 1965 Dec;3(3):73-85. doi: 10.3347/kjp.1965.3.3.73.
The mineral contents of the adult Clonorchis sinensis from rabbits and human were measured, and its qualitative and semi-quantitative analyses were studied with 3.4 meter Ebert-Grating spectrograph, and its quantitative analyses were studied with Beckman DU spectrophotometer. The adult Clonorchis sinensis used in this study were divided into two groups, the first group was collected from the bile passage of the man (C. sinensis from man), the second group was collected from the bile passage of the rabbit(C. sinensis from rabbit). Simultaneously, spectrographic and photometric analyses were also performed on the adult worms of Paragonimus westermani. Furthermore, comparative spectrographic analyses of the trace elements were carried out on the C. sinensis from man, C. sinensis from rabbit, Fasciola hepatica, Eurytrema pancreaticum, and Paragonimus westermani, and the approximate contents of the trace elements of the above trematodes were compared with that of their host tissues and biles. The results obtained were as follows: 1. In the spectrographic analyses of C. sinensis from man, sodium, phosphorus, potassium, copper, calcium, aluminum, iron, and magnesium were detected predominantly, and zinc, titanium, silicone, lead, manganese, barium, cromium, molybdenum, and silver were detected as trace elements. 2. In the differences of level of the minerals in these two C. sinensis, copper level of C. sinensis from man was higher than that of C. sinensis from rabbit, while calcium level of the former worm was higher than that of the latter worm. The trace level of lead, molybdenum, and silver were acertained in the former, but latter were not detected. 3. The contents of the minerals showed the characteristic features in each trematodes: the mineral of flukes in each host were much more than that of the others; such as copper in C. sinensis from man, calcium in the C. sinensis from rabbit, and silicone in the P. westermani. The vanadium was detected in the F. hepatica, E. pancreaticum, and P. westermani, while the other flukes were not detected. 4. In the comparative spectrographic analyses of the trace elements among the trematodes and their host tissues and biles, the minerals which detected from flukes were also found in their tissues and biles of their host. But the mineral levels of C. sinensis from man, F. hepatica, E. pancreaticum, and P. westermani were higher than that of their host tissues and biles, except the C. sinensis from rabbit.
测定了家兔和人体华支睾吸虫成虫的矿物质含量，并用3.4米埃伯特光栅光谱仪对其进行了定性和半定量分析，用贝克曼DU分光光度计对其进行了定量分析。本研究中使用的华支睾吸虫成虫分为两组，第一组从人体胆管收集（人体华支睾吸虫），第二组从兔胆管收集（兔体华支睾吸虫）。同时，对卫氏并殖吸虫成虫也进行了光谱和光度分析。此外，还对人体华支睾吸虫、兔体华支睾吸虫、肝片吸虫、胰阔盘吸虫和卫氏并殖吸虫进行了微量元素的比较光谱分析，并将上述吸虫的微量元素大致含量与其宿主组织和胆汁的含量进行了比较。结果如下：1. 在人体华支睾吸虫的光谱分析中，主要检测到钠、磷、钾、铜、钙、铝、铁和镁，锌、钛、硅、铅、锰、钡、铬、钼和银作为微量元素被检测到。2. 在这两种华支睾吸虫的矿物质水平差异中，人体华支睾吸虫的铜含量高于兔体华支睾吸虫，而前者的钙含量高于后者。在前者中确定了铅、钼和银的痕量水平，而在后者中未检测到。3. 矿物质含量在每种吸虫中表现出特征：每种宿主中的吸虫矿物质含量比其他吸虫多得多；例如人体华支睾吸虫中的铜、兔体华支睾吸虫中的钙和卫氏并殖吸虫中的硅。在肝片吸虫、胰阔盘吸虫和卫氏并殖吸虫中检测到钒，而在其他吸虫中未检测到。4. 在吸虫及其宿主组织和胆汁的微量元素比较光谱分析中，在吸虫中检测到的矿物质也在其宿主的组织和胆汁中发现。但是人体华支睾吸虫、肝片吸虫、胰阔盘吸虫和卫氏并殖吸虫的矿物质水平高于其宿主组织和胆汁，兔体华支睾吸虫除外。
