Ogasawara Yumie, Hiruma Masataro, Muto Masahiko
Department of Dermatology and Biomolecular Recognition,Yamaguchi University School of Medicine,1-1-1 Minami-Kogushi, Ube, Yamaguchi, 755-8505 Japan.
Nihon Ishinkin Gakkai Zasshi. 2003;44(3):217-20. doi: 10.3314/jjmm.44.217.
Pathogenic fungi can be identified by growth in special culture medium, colony appearance based on microscopic examination of conidia and hyphae, and by molecular biological techniques. However, these methods are time-consuming and labor-intensive. We describe a simple method for identification of pathogenic fungi from Sabouraud's glucose agar slant culture, using Fungi-Tape and MycoPerm-Blue. It is rapid and easy, and permits visualization of fungal morphology. Using the Fungi-Tape and MycoPerm-Blue technique, we correctly identified 47 of 66 samples, for a 73.4% concordance with conventional identification methods. It is also noteworthy that slides prepared using this method can be stored for long periods without deterioration.
致病性真菌可通过在特殊培养基中生长、基于分生孢子和菌丝体显微镜检查的菌落外观以及分子生物学技术来鉴定。然而，这些方法既耗时又费力。我们描述了一种使用真菌胶带（Fungi-Tape）和真菌渗透蓝（MycoPerm-Blue）从沙保弱葡萄糖琼脂斜面培养物中鉴定致病性真菌的简单方法。该方法快速简便，能够观察到真菌形态。使用真菌胶带和真菌渗透蓝技术，我们在66个样本中正确鉴定出47个，与传统鉴定方法的一致性为73.4%。同样值得注意的是，用这种方法制备的玻片可以长期保存而不会变质。
