Trevors J T
Department of Environmental Biology, University of Guelph, Guelph, ON N1G 2W1, Canada.
J Biochem Biophys Methods. 2003 Aug 29;57(2):87-103. doi: 10.1016/s0165-022x(03)00076-9.
Fluorescent methods in biological and medical research are extremely useful at the cellular and molecular levels. This is due to sensitive and affordable detection equipment and a variety of specific and more general fluorescent probes, and analytical procedures. In this article, I examine the use of fluorescence membrane probes to study the fluidity (membrane polarization) of the bacterial cytoplasmic membrane, central to energy transduction, ion and nutrient transport and diffusion of water and gases.
荧光方法在生物和医学研究中在细胞和分子水平上极为有用。这得益于灵敏且价格合理的检测设备、各种各样特异性和一般性更强的荧光探针以及分析程序。在本文中，我探讨了荧光膜探针在研究细菌细胞质膜流动性（膜极化）方面的应用，这种流动性对于能量转换、离子与营养物质运输以及水和气体的扩散至关重要。
