Moore Robert Y, Whone Alan L, McGowan Stephen, Brooks David J
Division of Neuroscience and MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College, Hammersmith Hospital, DuCane Road, London W12 ONN, UK.
Brain Res. 2003 Aug 29;982(2):137-45. doi: 10.1016/s0006-8993(03)02721-5.
18F-DOPA positron emission tomography (PET) has been used for two decades to study the organization and pathology of the striatal dopamine system in the human brain, particularly in Parkinson's disease. High resolution 3D PET allows a more detailed analysis than previously available and was employed in this study to determine the regional uptake of 18F-DOPA in control brain. Eleven healthy volunteers underwent 18F-DOPA PET with a region of interest (ROI) study performed using individual volumetric MRI's coregistered to the PET ADD image. A Patlak linear graphical analysis was undertaken to obtain influx constant (Ki) values. The highest Ki values were from neostriatal areas, with a rostrocaudal gradient of increasing Ki values from head of caudate nucleus to rostral putamen to caudal putamen. However, Ki values for transaxial slices from dorsal to ventral through the caudate and putamen were uniform. Ventral striatum Ki was 81% with red nucleus and globus pallidus Ki values of approximately 40% of neostriatum. In limbic areas, highest values were obtained from amygdala (35% neostriatal Ki). Neocortical Ki values varied from 22% in temporal pole to 6% in occipital cortex of neostriatal values. Hypothalamic Ki was high (45%) in comparison to thalamus (17%) and retina (17%). 18F-DOPA is taken up by serotonin (raphe, 51%), and noradrenaline (locus coeruleus, 37%) as well as dopamine neurons. These data indicate that 18F-DOPA PET can be used with detailed, anatomically based ROIs as a tool for in vivo analysis of regional changes in monoamine neuron systems throughout the brain in Parkinson's disease and other disorders.
18F - 多巴正电子发射断层扫描（PET）已被用于研究人类大脑纹状体多巴胺系统的组织和病理学长达二十年，尤其是在帕金森病研究中。高分辨率三维PET能够进行比以往更详细的分析，本研究采用该技术来确定对照大脑中18F - 多巴的区域摄取情况。11名健康志愿者接受了18F - 多巴PET检查，并使用与PET ADD图像配准的个体容积MRI进行了感兴趣区域（ROI）研究。采用Patlak线性图形分析来获取流入常数（Ki）值。最高的Ki值来自新纹状体区域，从尾状核头部到壳核前部再到壳核后部，Ki值呈头尾方向递增梯度。然而，通过尾状核和壳核从背侧到腹侧的横断面切片的Ki值是均匀的。腹侧纹状体的Ki值为81%，红核和苍白球的Ki值约为新纹状体的40%。在边缘系统区域，杏仁核获得了最高值（新纹状体Ki值的35%）。新皮质的Ki值在颞极相当于新纹状体值的22%，在枕叶皮质则为6%。与丘脑（17%）和视网膜（17%）相比，下丘脑的Ki值较高（45%）。18F - 多巴被5 - 羟色胺（中缝核，51%）、去甲肾上腺素（蓝斑，37%）以及多巴胺神经元摄取。这些数据表明，18F - 多巴PET可与基于详细解剖结构的ROI一起用作工具，用于体内分析帕金森病和其他疾病中全脑单胺神经元系统的区域变化。
