Segerman Anna, Atkinson John P, Marttila Marko, Dennerquist Veronica, Wadell Göran, Arnberg Niklas
Department of Virology, Umeå University, SE-90185 Umeå, Sweden.
J Virol. 2003 Sep;77(17):9183-91. doi: 10.1128/jvi.77.17.9183-9191.2003.
The 51 human adenovirus serotypes are divided into six species (A to F). Many adenoviruses use the coxsackie-adenovirus receptor (CAR) for attachment to host cells in vitro. Species B adenoviruses do not compete with CAR-binding serotypes for binding to host cells, and it has been suggested that species B adenoviruses use a receptor other than CAR. Species B adenoviruses mainly cause disease in the respiratory tract, the eyes, and in the urinary tract. Here we demonstrate that adenovirus type 11 (Ad11; of species B) binds to Chinese hamster ovary (CHO) cells transfected with CD46 (membrane cofactor protein)-cDNA at least 10 times more strongly than to CHO cells transfected with cDNAs encoding CAR or CD55 (decay accelerating factor). Nonpermissive CHO cells were rendered permissive to Ad11 infection upon transfection with CD46-cDNA. Soluble Ad11 fiber knob but not Ad7 or Ad5 knob inhibited binding of Ad11 virions to CD46-transfected cells, and anti-CD46 antibodies inhibited both binding of and infection by Ad11. From these results we conclude that CD46 is a cellular receptor for Ad11.
51种人腺病毒血清型分为六个种（A至F）。许多腺病毒在体外利用柯萨奇病毒-腺病毒受体（CAR）附着于宿主细胞。B种腺病毒不与结合CAR的血清型竞争与宿主细胞的结合，有人提出B种腺病毒使用CAR以外的受体。B种腺病毒主要引起呼吸道、眼睛和泌尿道疾病。在此我们证明，11型腺病毒（Ad11；B种）与转染了CD46（膜辅因子蛋白）-cDNA的中国仓鼠卵巢（CHO）细胞的结合力比与转染了编码CAR或CD55（衰变加速因子）cDNA的CHO细胞的结合力强至少10倍。用CD46-cDNA转染后，非允许性CHO细胞变得允许Ad11感染。可溶性Ad11纤维结蛋白而非Ad7或Ad5结蛋白抑制Ad11病毒粒子与转染了CD46的细胞的结合，抗CD46抗体抑制Ad11的结合和感染。从这些结果我们得出结论，CD46是Ad11的细胞受体。
