通过引物引入限制性酶切分析稻瘟病菌中细胞松弛素脱水酶基因来诊断黑色素生物合成抑制剂（MBI-D）抗性中的脱水酶抑制剂。
Diagnosis of dehydratase inhibitors in melanin biosynthesis inhibitor (MBI-D) resistance by primer-introduced restriction enzyme analysis in scytalone dehydratase gene of Magnaporthe grisea.
作者信息
Kaku Koichiro, Takagaki Makiichi, Shimizu Tsutomu, Nagayama Kozo
机构信息
Kumiai Chemical Industry Co Ltd, Life Science Research Institute, 3360 Kamo, Kikugawa, 439-0031 Japan.
出版信息
Pest Manag Sci. 2003 Aug;59(8):843-6. doi: 10.1002/ps.742.
DOI:10.1002/ps.742
PMID:12916764
Abstract
We have established a simple diagnosis method for rice blast fungus resistant to MBI-D. This involves the preparation of PCR templates directly from the lesions in combination with primer-introduced restriction enzyme analysis PCR (PIRA-PCR).
摘要
我们已经建立了一种针对对MBI-D具有抗性的稻瘟病菌的简单诊断方法。这涉及直接从病斑制备PCR模板，并结合引物引入的限制性酶切分析PCR（PIRA-PCR）。
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