薯蓣皂苷元B体外诱导人慢性髓系白血病K562细胞凋亡的研究

[Apoptosis of human chronic myeloid leukemia k562 cell induced by prosapogenin B of dioscin (P.B) in vitro].

作者信息

Wang San-Long, Cai Bing, Cui Cheng-Bin, Liu Hong-Wei, Wu Chun-Fu, Yao Xin-Sheng

机构信息

Tianjin Institute for Biomedicinal Research(TIBiR), Tianjin, 300384, PR China.

出版信息

Ai Zheng. 2003 Aug;22(8):795-800.

PMID:12917022

Abstract

BACKGROUND & OBJECTIVE: The authors have isolated prosapogenin B of dioscin (P.B) newly from Dioscorea futschauensis R. Kunth (Dioscoreaceae) and found that P.B could significantly inhibit the cell proliferation of several human cancer cell lines for the first time. The objective of this paper was designed to investigate whether P.B could inhibit cancer cell proliferation by inducing apoptosis.

METHODS

Morphological changes of cell nuclei were observed under invert fluorescence microscope and transmission electron microscope. The changes in the cell size distribution were analyzed by Coulter Multisizer II particle analyzer. DNA ladder and apoptotic cells were analyzed respectively by DNA agarose gel electrophoresis and flow cytometry.

RESULTS

The K562 cells treated with 10 micromol/L P.B for 24 hours showed morphological characteristics of apoptotic cells, such as decrease in cellular volume, nuclear chromatin condensation, nuclear fragmentation, and plasma membrane bleb formation. The treatment of K562 cells with 10 micromol/L P.B for 6, 12,and 24 hours caused the increase of the detected DNA ladder and the decrease of normal size cells both in a time-dependent manner. The percentage of apoptotic bodies detected in the sub-G(0)/G(1) peak region by flow cytometry was also increased time-dependently as analyzed as 6.12% for 6 hours, 35.6% for 12 hours and 45.7% for 24 hours treatments, respectively.

CONCLUSION

These results suggested that P.B exerts its anti-proliferative effect on K562 cells through inducing apoptosis of the cells.

摘要

背景与目的

作者从黄山药（薯蓣科）中首次新分离到薯蓣皂苷元薯蓣皂苷原B（P.B），并发现P.B能显著抑制多种人癌细胞系的细胞增殖。本文旨在研究P.B是否通过诱导凋亡来抑制癌细胞增殖。

方法

在倒置荧光显微镜和透射电子显微镜下观察细胞核的形态变化。用库尔特多参数血细胞分析仪分析细胞大小分布的变化。分别用DNA琼脂糖凝胶电泳和流式细胞术分析DNA梯带和凋亡细胞。

结果

用10 μmol/L P.B处理K562细胞24小时后，细胞呈现凋亡特征，如细胞体积减小、核染色质凝聚、核碎裂和质膜泡形成。用10 μmol/L P.B处理K562细胞6、12和24小时，均可使检测到的DNA梯带增加，正常大小细胞减少，且均呈时间依赖性。流式细胞术分析显示，在亚G(0)/G(1)峰区域检测到的凋亡小体百分比也随时间增加，6小时处理时为6.12%，12小时处理时为35.6%，24小时处理时为45.7%。