榄香烯诱导人白血病K562细胞凋亡并调节bcl-2蛋白的表达。
Elemene induces apoptosis and regulates expression of bcl-2 protein in human leukemia K562 cells.
作者信息
Yuan J, Gu Z L, Chou W H, Kwok C Y
机构信息
Department of Pharmacology, Suzhou Medical College, China.
出版信息
Zhongguo Yao Li Xue Bao. 1999 Feb;20(2):103-6.
AIM
To study the antitumor action of elemene (Ele) and its mechanism.
METHODS
Inhibition of proliferation was measured with a colorimetric 3-[4,5-dimethyl thiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Morphological assessment of apoptosis was performed with fluorescence microscope. DNA fragmentation was assessed by agarose gel electrophoresis and flow cytometry. The levels of bcl-2 protein was measured with flow cytometry.
RESULTS
Exposure of exponentially growing K562 cells to Ele 65-520 mumol. L-1 for 48 h resulted in growth arrest. The values of IC50 and 95% confidence limits were 220 (152-319) mumol.L-1. After treatment of K562 cells with Ele 130 mumol.L-1, marked morphological changes including "Apo bodies" reduction in volume were observed with fluorescence microscope. Agarose gel electrophoresis of DNA from cells treated with Ele for 48 h revealed "ladder" pattern. The levels of bcl-2 protein in K562 cells treated with Ele for 48 h were obviously decreased.
CONCLUSION
Ele induces apoptosis of K562 cells, which is related with the down-regulation of bcl-2 protein in K562 cells.
目的
研究榄香烯（Ele）的抗肿瘤作用及其机制。
方法
采用比色法3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）法检测细胞增殖抑制率。用荧光显微镜对细胞凋亡进行形态学评估。通过琼脂糖凝胶电泳和流式细胞术检测DNA片段化情况。用流式细胞术检测bcl-2蛋白水平。
结果
对数生长期的K562细胞暴露于65 - 520μmol·L-1的Ele中48小时后出现生长停滞。IC50值及95%置信区间为220（152 - 319）μmol·L-1。用130μmol·L-1的Ele处理K562细胞后，荧光显微镜下观察到明显的形态学变化，包括“凋亡小体”体积减小。对经Ele处理48小时的细胞DNA进行琼脂糖凝胶电泳，呈现出“梯状”条带。经Ele处理48小时的K562细胞中bcl-2蛋白水平明显降低。
结论
榄香烯诱导K562细胞凋亡，这与K562细胞中bcl-2蛋白表达下调有关。
Zotero 插件