Jankovic Mila, Nussenzweig Michel C
Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021-6399, USA.
Int Immunol. 2003 Sep;15(9):1099-104. doi: 10.1093/intimm/dxg109.
OcaB, also known as Bob-1 or Obf-1, is a transcriptional co-activator which regulates Igkappa gene transcription, recombination and receptor editing; it is required for normal development of transitional B cells and for germinal center formation. Here we report that abnormal B cell development in OcaB(-/-) mice results in a skewed Igkappa repertoire including anti-DNA antibodies, suggesting that OcaB is essential for antibody repertoire selection. To determine whether OcaB is required for BCR-mediated B cell selection, we introduced a pre-recombined alpha hen egg lysozyme (HEL) Ig transgene into OcaB(-/-) mice. We find that in OcaB(-/-) mice expressing transgenic alphaHEL Ig bone marrow B cell development is normal up to the immature B cell stage, but fails to progress to the transitional B cell stage. We conclude that OcaB is required for normal selection of the antibody repertoire in developing B cells.
OcaB，也被称为Bob-1或Obf-1，是一种转录共激活因子，可调节Igκ基因的转录、重组和受体编辑；它是过渡性B细胞正常发育和生发中心形成所必需的。在此我们报告，OcaB基因敲除小鼠中异常的B细胞发育导致包括抗DNA抗体在内的Igκ库失衡，这表明OcaB对于抗体库选择至关重要。为了确定OcaB是否是BCR介导的B细胞选择所必需的，我们将一个预重组的α-鸡卵溶菌酶（HEL）Ig转基因导入OcaB基因敲除小鼠。我们发现，在表达转基因αHEL Ig的OcaB基因敲除小鼠中，骨髓B细胞发育在未成熟B细胞阶段之前是正常的，但无法进展到过渡性B细胞阶段。我们得出结论，OcaB是发育中的B细胞正常选择抗体库所必需的。
