胰腺癌的细胞核形态测定、DNA含量与可切除性之间的关系。

Relationship between nuclear morphometry, DNA content and resectability of pancreatic cancer.

作者信息

He Yin-Cheng, Peng Wei, Qiao Jian-Guo, Cao Jun, Chen Ji-Wei

机构信息

Department of General Surgery, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, China.

出版信息

World J Gastroenterol. 2003 Aug;9(8):1863-5. doi: 10.3748/wjg.v9.i8.1863.

DOI:10.3748/wjg.v9.i8.1863

PMID:12918140

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4611563/

Abstract

AIM

To investigate the association of nuclear morphometry and DNA content with resectability of pancreatic cancer.

METHODS

A total of 36 patients with pancreatic adenocarcinoma were divided into resectable group and unresectable group. The nuclear morphometry and DNA contents of tumor cells were analyzed by IBAS autoimagine analyzer from paraffin-embedded materials. Localization size, histological type and grade, and clinical stage of the tumor were evaluated. Factors influencing resectability of pancreatic cancer were investigated using stepwise regression analysis.

RESULTS

Statistical significance was found in nuclear DNA content (integrated optical density, IOD) of tumor cells (1.64+/-0.41 vs 2.96+/-0.55), DNA ploidy, ages (46.5+/-5.3 years vs 58.6+/-0.7 years) and tumor volumes (298.1+/-101.5 cm(3) vs 634.7+/-512.5 cm(3)) in both groups (P<0.05), and no difference was found in the nuclear morphometry (P>0.05). The rates of diploid/tetraploid and aneuploid were 66.7 % and 33.3 % in resectable group respectively, and 38.9 % and 62.1 % in unresectable group, respectively (P<0.05). IOD (X(12)), ploidy status (X(12)) and clinical stage (X(3)) were radical resectable indicators with statistical significance. The regression equation for resectability was Y=-9.2053+3.5428X(12)+2.5390X(13)-2.3001X(3) (RR=0.8780, P<0.01).

CONCLUSION

There is a high correlation between resectability of pancreatic cancers and their DNA contents, DNA ploidy status and clinical stage.

摘要

目的

探讨细胞核形态计量学及DNA含量与胰腺癌可切除性之间的关系。

方法

36例胰腺腺癌患者被分为可切除组和不可切除组。采用IBAS自动图像分析仪对石蜡包埋材料中的肿瘤细胞核形态计量学及DNA含量进行分析。评估肿瘤的定位大小、组织学类型和分级以及临床分期。采用逐步回归分析研究影响胰腺癌可切除性的因素。

结果

两组肿瘤细胞核DNA含量（积分光密度，IOD）（1.64±0.41对2.96±0.55）、DNA倍体、年龄（46.5±5.3岁对58.6±0.7岁）和肿瘤体积（298.1±101.5cm³对634.7±512.5cm³）差异有统计学意义（P<0.05），而细胞核形态计量学无差异（P>0.05）。可切除组二倍体/四倍体和非整倍体率分别为66.7%和33.3%，不可切除组分别为38.9%和62.1%（P<0.05）。IOD（X₁₂）、倍体状态（X₁₂）和临床分期（X₃）是具有统计学意义的根治性可切除指标。可切除性的回归方程为Y=-9.2053+3.5428X₁₂+2.5390X₁₃-2.3001X₃（RR=0.8780，P<0.01）。