Ovsyannikova Inna G, Johnson Kenneth L, Naylor Stephen, Muddiman David C, Poland Gregory A
Mayo Vaccine Research Group, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
Virology. 2003 Aug 1;312(2):495-506. doi: 10.1016/s0042-6822(03)00281-2.
This is the first report of the direct identification of a HLA-DRB1*03 measles-derived peptide from measles virus infected EBV-transformed B cells. We purified HLA-DR3-peptide complexes from EBV-B cells infected with measles virus (Edmonston strain) and sequenced the HLA-DR3-peptides by mass spectrometry. A class II peptide, derived from a measles phosphoprotein, ASDVETAEGGEIHELLRLQ (P1, residues 179-197), exhibited the capacity to stimulate peripheral blood mononuclear cells to proliferate. Our data provides direct evidence that the antigenic peptide of measles virus was processed by antigen-presenting cells, presented in the context of HLA class II molecules, and was recognized by peripheral blood T cells from healthy individuals previously immunized with measles vaccine. The approach described herein provides a useful methodology for the future identification of HLA-presented pathogen-derived epitopes using mass spectrometry. The study of cell-mediated immune responses to the measles-derived peptide in immune persons should provide significant insight into the design and development of new vaccines.
这是首次直接鉴定出源自麻疹病毒感染的EB病毒转化B细胞的HLA - DRB1*03麻疹衍生肽的报告。我们从感染麻疹病毒（埃德蒙斯顿株）的EB病毒B细胞中纯化了HLA - DR3 - 肽复合物，并通过质谱对HLA - DR3 - 肽进行测序。一种源自麻疹磷蛋白的II类肽ASDVETAEGGEIHELLRLQ（P1，第179 - 197位氨基酸残基）表现出刺激外周血单个核细胞增殖的能力。我们的数据提供了直接证据，表明麻疹病毒的抗原肽由抗原呈递细胞加工处理，在HLA II类分子的背景下呈递，并被先前接种过麻疹疫苗的健康个体的外周血T细胞识别。本文所述方法为未来使用质谱鉴定HLA呈递的病原体衍生表位提供了一种有用的方法。对免疫个体中针对麻疹衍生肽的细胞介导免疫反应的研究应为新型疫苗的设计和开发提供重要见解。
