Cheng Meng-Dawn, Malone Boyd, Storey John M E
Environmental Sciences Division, Oak Ridge National Laboratory, PO Box 2008, MS 6038, Oak Ridge, TN 37831-6038, USA.
Chemosphere. 2003 Oct;53(3):237-43. doi: 10.1016/S0045-6535(03)00555-1.
The impacts of ultrafine airborne particles generated by diesel or gasoline engines on human lung cells have been investigated using a new in vitro cellular exposure technique. This technique enables direct deposition of the gasoline engine exhaust particles (GEP) and diesel engine exhaust particles (DEP) on human lung cells located at the air-cell interface on a transwell membrane in an exposure apparatus. The cellular responses to particle exposure were measured by the levels of IL-8 chemokines produced as a function of exposure time. The findings suggest that GEP and high-sulfur DEP induced the production of similar levels of IL-8 by unprimed A549 cells. The level of IL-8 produced by unprimed A549 cells in response to low-sulfur DEP was found lower than that produced in response to high-sulfur DEP and GEP. When cells were primed, simulating predisposed conditions, significant levels of IL-8 were produced. GEP triggered a much higher level of IL-8 production than DEP did. Furthermore, the time profile of IL-8 production induced by GEP was markedly different from that induced by DEP. The findings indicate that GEP could induce the production of higher levels of chemokines (i.e., IL-8) than DEP did, implying that exposure to GEP could be a greater health risk than exposure to DEP.
利用一种新的体外细胞暴露技术，研究了柴油发动机或汽油发动机产生的超细空气传播颗粒对人肺细胞的影响。该技术能够使汽油发动机尾气颗粒（GEP）和柴油发动机尾气颗粒（DEP）直接沉积在暴露装置中跨膜小室气液界面处的人肺细胞上。通过作为暴露时间函数产生的IL-8趋化因子水平来测量细胞对颗粒暴露的反应。研究结果表明，未预处理的A549细胞对GEP和高硫DEP诱导产生的IL-8水平相似。发现未预处理的A549细胞对低硫DEP产生的IL-8水平低于对高硫DEP和GEP产生的IL-8水平。当细胞被预处理以模拟易感条件时，会产生显著水平的IL-8。GEP引发的IL-8产生水平比DEP高得多。此外，GEP诱导的IL-8产生的时间曲线与DEP诱导的明显不同。研究结果表明，GEP比DEP能诱导产生更高水平的趋化因子（即IL-8），这意味着暴露于GEP可能比暴露于DEP带来更大的健康风险。
