Frew T, Whitaker B, Salter D, Payne W, Hughes S, Yamamoto H, Takeuchi T, Brumbaugh J
School of Biological Sciences, University of Nebraska, Lincoln 68588-0118.
Pigment Cell Res. 1992 Nov;5(5 Pt 2):312-21. doi: 10.1111/j.1600-0749.1992.tb00555.x.
Virally introduced mouse tyrosinase expression was checked both in vitro and in vivo in chicken cells and tissues. The results indicate that a constitutive promoter is able to express mouse tyrosinase in a variety of cells and tissues both in vitro and in vivo. Tyrosinase expression is marked by pigment production in situ, which is visible at macroscopic as well as microscopic levels without the use of substrates. It is concluded that tyrosinase can be a valuable marker for tracking gene insertion since it is spontaneously expressed. The expression of tyrosinase in some cells and tissues has a detrimental effect, however, and should be controlled by tissue-specific promoters.
在鸡的细胞和组织中，对病毒导入的小鼠酪氨酸酶表达进行了体内和体外检测。结果表明，组成型启动子能够在体外和体内的多种细胞和组织中表达小鼠酪氨酸酶。酪氨酸酶表达的标志是原位产生色素，无需使用底物，在宏观和微观水平均可看到。结论是，由于酪氨酸酶能自发表达，它可作为追踪基因插入的一个有价值的标志物。然而，酪氨酸酶在某些细胞和组织中的表达具有有害作用，应通过组织特异性启动子进行控制。
