Song Hui-feng, Chai Jia-ke, Lin Zi-hao
Department of Burn and Plastic Surgery, 304th Hospital, Beijing, P. R. China, 100037.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2003 Jul;17(4):298-300.
To explore the expression of basic fibroblast growth factor(bFGF) during the wound healing of human fetal and adult skin and its significance.
We established the animal model of fetal scarless healing by transplanting full-thickness skin grafts from human fetus to a subcutaneous location on the athymic mouse recipient, and then making the linear incisions. The expression of bFGF was observed in the normal adult skin, normal fetal skin and during wound healing by immunohistochemical method. The positive staining cells were counted under selected high-power focus randomly.
bFGF staining was not observed in the normal fetal skin and the wounded one. However, strongly positive staining was shown around the vessels in normal adult skin. Moreover, the positive straining became stronger in the wounded skin, especially in dermal fibroblasts and endotheliocytes. The number of positive staining cell was 2.1 +/- 0.1 in normal fetal skin, and 2.2 +/- 0.1, 2.1 +/- 0.3, 2.1 +/- 0.3 and 2.0 +/- 0.1 in the fetal skins after 12 hours, 1 day, 3 days and 7 days of wound respectively. The number of positive staining cell were 23.2 +/- 4.2 in normal adult skin and 40.5 +/- 3.6 in the wound adult skin. There was significant difference between the fetal skin and adult skin (P < 0.01).
The negative expression of bFGF in the fetal skin may be one of the important reasons for fetal scarless healing.
探讨碱性成纤维细胞生长因子（bFGF）在人胎儿及成人皮肤伤口愈合过程中的表达及其意义。
通过将人胎儿全层皮肤移植到无胸腺小鼠受体的皮下部位，然后制作线性切口，建立胎儿无瘢痕愈合的动物模型。采用免疫组织化学方法观察bFGF在正常成人皮肤、正常胎儿皮肤及伤口愈合过程中的表达。在选定的高倍视野下随机计数阳性染色细胞。
在正常胎儿皮肤及受伤胎儿皮肤中未观察到bFGF染色。然而，在正常成人皮肤的血管周围显示强阳性染色。此外，在受伤皮肤中阳性染色更强，尤其是在真皮成纤维细胞和内皮细胞中。正常胎儿皮肤中阳性染色细胞数为2.1±0.1，伤口后12小时、1天、3天和7天的胎儿皮肤中分别为2.2±0.1、2.1±0.3、2.1±0.3和2.0±0.1。正常成人皮肤中阳性染色细胞数为23.2±4.2，受伤成人皮肤中为40.5±3.6。胎儿皮肤和成人皮肤之间存在显著差异（P<0.01）。
胎儿皮肤中bFGF的阴性表达可能是胎儿无瘢痕愈合的重要原因之一。
