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[重金属胁迫下土壤微生物及其介导过程的研究进展]

[Advances in the research of soil microorganisms and their mediated processes under heavy metal stress].

作者信息

Yu Shen, He Zhenli, Huang Changyong

机构信息

School of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310029, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2003 Apr;14(4):618-22.

Abstract

More and more evidence from both laboratory and field experiments suggested that soil microbes were more sensitive to heavy metal stress than soil animals or plants living in the same soil. This understanding has been gradually accepted as a common point in this field. The history and the latest advances in soil microorganisms and its mediated processes under heavy metal stresses in soils were reviewed in this paper. It is difficult to do any comparison among the results from laboratory, field toxicological experiment and environmental monitoring. So far, a relevantly 'accurate' control soil was rarely available in the environmental monitoring. Much of evidence has indicated that not only microbial population/biomass was changed under heavy metal stress, but also its structure was varied. Although many molecular methods, such as PLFA, BIOLOG and DNA, have been developed to detect this microbial structural change, they always need many expensive instruments and accessories, and only could be done in laboratory. Therefore, some rapid and easy methods are expected to develop to substitute the traditional plate count. The speciation in the solution phase, ion effect and rhizospheric effect of heavy metal on the toxicity to soil microorganisms and its mediated processes need extent studies and discussion. Finally, the microbial indicator system of assessing heavy metal toxicity to soil microorganisms and its mediated processes need to be established.

摘要

越来越多来自实验室和田间试验的证据表明,与生活在同一土壤中的土壤动物或植物相比,土壤微生物对重金属胁迫更为敏感。这一认识已逐渐被该领域视为一个共识。本文综述了土壤微生物及其在土壤重金属胁迫下介导过程的研究历史和最新进展。实验室、田间毒理学试验和环境监测的结果之间很难进行比较。到目前为止,环境监测中很少有相对“准确”的对照土壤。许多证据表明,重金属胁迫下不仅微生物数量/生物量发生了变化,其结构也有所改变。虽然已经开发了许多分子方法,如磷脂脂肪酸分析(PLFA)、Biolog微平板分析和DNA分析来检测这种微生物结构变化,但它们总是需要许多昂贵的仪器和配件,并且只能在实验室中进行。因此,期望开发一些快速简便的方法来替代传统的平板计数法。重金属在溶液相中的形态、离子效应和根际效应及其对土壤微生物毒性及其介导过程的影响需要进一步研究和讨论。最后,需要建立评估重金属对土壤微生物毒性及其介导过程的微生物指示体系。

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