[下调的全长新基因GDDR及其对胃癌的影响]

[Down-regulated full-length novel gene GDDR and its effect on gastric cancer].

作者信息

Du Jian-jun, Dou Ke-feng, Peng Shu-you, Wang Wei-zhong, Wang Zhong-hua, Xiao Hua-sheng, Guan Wen-xian, Liu Ying-bin, Gao Zhi-qing

机构信息

Department of General Surgery, Xijing Hospital, The Fourth Military Medical University, Xian 710032, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2003 Jul 10;83(13):1166-8.

PMID:12921637

Abstract

OBJECTIVE

To clone gene fragments from suppression substructive library established for screening down-regulated genes in gastric carcinoma, and obtain the full-length novel gene.

METHODS

Gene fragments were identified by sequencing plasmids of positive colonies chosen randomly. One gene fragment was amplified by RACE, and the full-length novel gene was obtained. Expression of novel gene mRNA was respectively detected by semi-quantitative PCR in the gastric carcinoma tissues and counterpart normal gastric mucous membrane of 25 patients with gastric cancer. The structure of the full-length novel gene, location on chromosome, property of protein encoded by full-length novel gene and its function were investigated by Bio-message technique.

RESULTS

One 331 bp gene fragment was cloned, and its full-length novel gene obtained by RACE. The novel full-length gene was named GDDR, registered in the number of AF494509 by GenBank. Significant down-regulated expression of GDDR gene mRNA in gastric carcinoma tissues was confirmed (GDDR/beta-actin 13.474 +/- 5.059 vs 1.041 +/- 0.202, P < 0.01). GDDR was located in chromosome 2p13 with 5 exons. As one member of new BRICHO family as CA11, GDDR encoding protein with transmembrane peptide revealed homology to protein encoded by CA11.

CONCLUSION

A novel full-length gene GDDR is obtained. GDDR likely is another gene of BRICHO family related to gastric cancer.

摘要

目的

从为筛选胃癌下调基因而构建的抑制性消减文库中克隆基因片段，获得新基因全长。

方法

随机挑选阳性克隆质粒进行测序鉴定基因片段。采用RACE技术扩增其中一个基因片段，获得新基因全长。应用半定量PCR分别检测25例胃癌患者癌组织及其相应正常胃黏膜中该新基因mRNA的表达情况。利用生物信息学技术研究新基因全长的结构、染色体定位、全长新基因编码蛋白的性质及其功能。

结果

克隆到一个331bp的基因片段，通过RACE获得其全长新基因。该新全长基因命名为GDDR，在GenBank注册登记号为AF494509。证实GDDR基因mRNA在胃癌组织中表达明显下调（GDDR/β-肌动蛋白：13.474±5.059 vs 1.041±0.202，P<0.01）。GDDR定位于染色体2p13，含5个外显子。作为新BRICHO家族成员之一，与CA11类似，GDDR编码的蛋白含跨膜肽段，与CA11编码的蛋白具有同源性。