[缺血预处理对肺缺血再灌注损伤期间细胞因子的影响]

[Effect of ischemic preconditioning on cytokines during lung ischemia-reperfusion injury].

作者信息

Zhang Tao, Zhang Feng, Zhou Yong-an, Wang Yun-jie, Li Xiao-fei, Chen De-feng

机构信息

Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.

出版信息

Zhonghua Wai Ke Za Zhi. 2003 Jul;41(7):545-7.

PMID:12921666

Abstract

OBJECTIVE

To investigate the protective role of ischemic preconditioning (IPC) during lung ischemia-reperfusion (I/R) injury and its influence on inflammatory cytokine production.

METHODS

In vivo I/R injury of rabbit was induced by blocking hilum of the left lung. The wet/dry ratio of the lung, lung permeability index and neutrophils percentage in bronchoalveolar lavage fluid (BALF) were detected as indexes of the lung injury. Serum levels of tumor necrosis factor alpha (TNFalpha), interleukin-6 (IL-6) and interleukin-8 (IL-8) were also detected using enzyme-linked immunosorbent assay. The protective role of IPC and its influence on inflammatory cytokine production were observed.

RESULTS

The wet/dry ratio of the lung, lung permeability index and neutrophils percentage in BALF of I/R group were 9.73 +/- 1.14, (41.62 +/- 5.77) x 10(-4) and (58.1 +/- 10.0)% respectively. The IPC group indexes were 6.23 +/- 0.69, (20.31 +/- 4.03) x 10(-4) and (23.8 +/- 5.2)% respectively. There was a significant difference between the two groups (P < 0.01). Serum levels of TNFalpha, IL-6 and IL-8 of I/R group were (0.9078 +/- 0.1062), (0.2137 +/- 0.0598) and (0.7211 +/- 0.0979) ng/ml respectively. The IPC group indexes were (0.7478 +/- 0.0843), (0.1271 +/- 0.0089) and (0.5903 +/- 0.0746) ng/ml respectively, significantly lower than that of I/R group (P < 0.01).

CONCLUSIONS

Lung IPC has a marked protection effect against I/R injury. The effect was related to its inhibition of inflammatory cytokines such as TNFalpha, IL-6 and IL-8, thus reducing activation and infiltration of neutrophils.

摘要

目的

探讨缺血预处理（IPC）在肺缺血再灌注（I/R）损伤中的保护作用及其对炎性细胞因子产生的影响。

方法

通过阻断兔左肺门诱导体内I/R损伤。检测肺组织湿/干比、肺通透性指数及支气管肺泡灌洗液（BALF）中中性粒细胞百分比作为肺损伤指标。采用酶联免疫吸附测定法检测血清肿瘤坏死因子α（TNFα）、白细胞介素-6（IL-6）和白细胞介素-8（IL-8）水平。观察IPC的保护作用及其对炎性细胞因子产生的影响。

结果

I/R组肺组织湿/干比、肺通透性指数及BALF中中性粒细胞百分比分别为9.73±1.14、（41.62±5.77）×10⁻⁴和（58.1±10.0）%。IPC组指标分别为6.23±0.69、（20.31±4.03）×10⁻⁴和（23.8±5.2）%。两组间差异有统计学意义（P<0.01）。I/R组血清TNFα、IL-6和IL-8水平分别为（0.9078±0.1062）、（0.2137±0.0598）和（0.7211±0.0979）ng/ml。IPC组指标分别为（0.7478±0.0843）、（0.1271±0.0089）和（0.5903±0.0746）ng/ml，明显低于I/R组（P<0.01）。