Nguyen T V, Iosef C, Jeong K, Kim Y, Chang K-O, Lovgren-Bengtsson K, Morein B, Azevedo M S P, Lewis P, Nielsen P, Yuan L, Saif L J
Food Animal Health Research Program, Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691-4096, USA.
Vaccine. 2003 Sep 8;21(25-26):4059-70. doi: 10.1016/s0264-410x(03)00267-6.
We evaluated antibody responses and protection induced by attenuated Wa human rotavirus (AttHRV) and VP2/6-rotavirus-like particles (VLP), 100 or 250 microg/dose, with immunostimulating complexes (ISCOM) (VLP/ISCOM) each given orally, alone or sequentially to gnotobiotic pigs. The AttHRV-VLP 250 microg/ISCOM and three-dose-AttHRV (AttHRV3x) groups had significantly higher serum IgA, IgG and intestinal IgA antibody titers to HRV pre-challenge than the three-dose-VLP 100 microg/ISCOM group (VLP/ISCOM3x) and controls (diluent/ISCOMmatrix). Protection rates against viral shedding and diarrhea were highest in the AttHRV-VLP250 microg/ISCOM and AttHRV3x groups, lower in the AttHRV-VLP 100 microg/ISCOM group, with no protection in the VLP/ISCOM3x group and controls. Thus, VLP/ISCOM boosted antibody titers and protection after priming with AttHRV.
我们评估了减毒Wa人轮状病毒（AttHRV）和VP2/6-轮状病毒样颗粒（VLP）（剂量分别为100或250微克）与免疫刺激复合物（ISCOM）（VLP/ISCOM）单独或先后经口给予无菌猪后诱导的抗体反应和保护作用。与三剂量100微克/ISCOM的VLP组（VLP/ISCOM3x）和对照组（稀释剂/ISCOM基质）相比，250微克/ISCOM的AttHRV-VLP组和三剂量AttHRV组（AttHRV3x）在攻击前对HRV的血清IgA、IgG和肠道IgA抗体滴度显著更高。AttHRV-VLP 250微克/ISCOM组和AttHRV3x组对病毒排泄和腹泻的保护率最高，AttHRV-VLP 100微克/ISCOM组较低，VLP/ISCOM3x组和对照组无保护作用。因此，VLP/ISCOM在用AttHRV进行初免后可提高抗体滴度和保护作用。
