Roos Andreas, Klee Doris, Schuermann Karl, Höcker Hartwig
Department of Textile Chemistry and Macromolecular Chemistry, RWTH Aachen, Veltmanplatz 8, 52062 Aachen, Germany.
Biomaterials. 2003 Nov;24(24):4417-23. doi: 10.1016/s0142-9612(03)00342-9.
A low density polyethylene (LDPE) model surface was coated with poly(amino-p-xylylene) (amino-ppx) via chemical vapour deposition (CVD) polymerization. The functional surface was used to immobilize a polymeric drug release system consisting of poly(N-isopropylacrylamide) (NIPAAm)-co-poly(acrylic acid) (AAc). The coupled drug release system was used to incorporate the thrombin inhibitor r-hirudin. For the investigation of the concentration of incorporated r-hirudin and the release profile over a given period of time r-hirudin was labelled with 123I as well as fluorescein isothiocyanate (FITC). Using an incubation solution concentration of 0.2% at pH 5 and an ionic strength of 0.7 M a maximum concentration of 2.21 (+/- 0.11) nmol/cm2 of r-hirudin was detected. FITC-r-hirudin was almost quantitatively (2.08 +/- 10 nmol/cm2; 94%) released from the surface coating within a period of 14 days.
通过化学气相沉积（CVD）聚合，在低密度聚乙烯（LDPE）模型表面涂覆聚（氨基对二甲苯）（氨基-ppx）。该功能化表面用于固定由聚（N-异丙基丙烯酰胺）（NIPAAm）-共聚（丙烯酸）（AAc）组成的聚合物药物释放系统。耦合的药物释放系统用于掺入凝血酶抑制剂重组水蛭素。为了研究掺入的重组水蛭素的浓度以及在给定时间段内的释放曲线，重组水蛭素用123I以及异硫氰酸荧光素（FITC）进行标记。在pH值为5、离子强度为0.7 M、孵育溶液浓度为0.2%的条件下，检测到重组水蛭素的最大浓度为2.21（±0.11）nmol/cm2。FITC-重组水蛭素在14天内几乎定量地（2.08±10 nmol/cm2；94%）从表面涂层释放。
