鼠疫耶尔森菌噬菌体phiA1122的基因组序列揭示了其与大肠杆菌噬菌体T3和T7基因组密切的演化历史。
The genome sequence of Yersinia pestis bacteriophage phiA1122 reveals an intimate history with the coliphage T3 and T7 genomes.
作者信息
Garcia Emilio, Elliott Jeffrey M, Ramanculov Erlan, Chain Patrick S G, Chu May C, Molineux Ian J
机构信息
Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA, USA.
出版信息
J Bacteriol. 2003 Sep;185(17):5248-62. doi: 10.1128/JB.185.17.5248-5262.2003.
The genome sequence of bacteriophage phiA1122 has been determined. phiA1122 grows on almost all isolates of Yersinia pestis and is used by the Centers for Disease Control and Prevention as a diagnostic agent for the causative agent of plague. phiA1122 is very closely related to coliphage T7; the two genomes are colinear, and the genome-wide level of nucleotide identity is about 89%. However, a quarter of the phiA1122 genome, one that includes about half of the morphogenetic and maturation functions, is significantly more closely related to coliphage T3 than to T7. It is proposed that the yersiniophage phiA1122 recombined with a close relative of the Y. enterocolitica phage phiYeO3-12 to yield progeny phages, one of which became the classic T3 coliphage of Demerec and Fano (M. Demerec and U. Fano, Genetics 30:119-136, 1945).
噬菌体phiA1122的基因组序列已被测定。phiA1122能在几乎所有鼠疫耶尔森菌分离株上生长，美国疾病控制与预防中心将其用作鼠疫病原体的诊断试剂。phiA1122与大肠杆菌噬菌体T7密切相关；这两个基因组是共线的，全基因组核苷酸同一性水平约为89%。然而，phiA1122基因组的四分之一，即包含约一半形态发生和成熟功能的部分，与大肠杆菌噬菌体T3的亲缘关系明显比与T7的更近。有人提出，鼠疫噬菌体phiA1122与小肠结肠炎耶尔森菌噬菌体phiYeO3 - 12的一个近亲发生重组，产生了子代噬菌体，其中一个子代噬菌体成为了Demerec和Fano经典的大肠杆菌噬菌体T3（M. Demerec和U. Fano，《遗传学》30:119 - 136，1945年）。
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