噬菌体T3的完整核苷酸序列及可能的重组起源

Complete nucleotide sequence and likely recombinatorial origin of bacteriophage T3.

作者信息

Pajunen Maria I, Elizondo Michael R, Skurnik Mikael, Kieleczawa Jan, Molineux Ian J

机构信息

Department of Medical Biochemistry and Molecular Biology, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, Finland.

出版信息

J Mol Biol. 2002 Jun 21;319(5):1115-32. doi: 10.1016/S0022-2836(02)00384-4.

DOI:10.1016/S0022-2836(02)00384-4

PMID:12079351

Abstract

We report the complete genome sequence (38,208 bp) of bacteriophage T3 and provide a bioinformatic comparative analysis with other completely sequenced members of the T7 group of phages. This comparison suggests that T3 has evolved from a recombinant between a T7-like coliphage and a yersiniophage. To assess this, recombination between T7 and the Yersinia enterocolitica serotype O:3 phage phiYeO3-12 was accomplished in vivo; coliphage progeny from this cross were selected that had many biological properties of T3. This represents the first experimentally observed recombination between lytic phages whose normal hosts are different bacterial genera.

摘要

我们报告了噬菌体T3的完整基因组序列（38,208 bp），并与T7噬菌体组中其他已完全测序的成员进行了生物信息学比较分析。该比较表明，T3是由一种T7样大肠杆菌噬菌体和一种耶尔森氏菌噬菌体之间的重组体进化而来。为了评估这一点，T7与小肠结肠炎耶尔森氏菌O:3血清型噬菌体phiYeO3-12之间的重组在体内完成；从该杂交中选择了具有T3许多生物学特性的大肠杆菌噬菌体后代。这代表了首次在实验中观察到正常宿主为不同细菌属的裂解性噬菌体之间的重组。