球形红细菌鞭毛基体的纯化与表征
Purification and characterization of the flagellar basal body of Rhodobacter sphaeroides.
作者信息
Kobayashi K, Saitoh T, Shah D S H, Ohnishi K, Goodfellow I G, Sockett R E, Aizawa S-I
机构信息
Department of Biosciences, Teikyo University, 1-1 Toyosatodai, Utsunomiya 320-8551, Japan.
出版信息
J Bacteriol. 2003 Sep;185(17):5295-300. doi: 10.1128/JB.185.17.5295-5300.2003.
Abstract
Flagellar hook-basal body (HBB) complexes were purified from Rhodobacter sphaeroides. The HBB was more acid labile but more heat stable than that of Salmonella species, and protein identification revealed that HBB components were expressed only from one of the two sets of flagellar gene clusters on the R. sphaeroides genome, under the heterotrophic growth conditions tested here.
摘要
鞭毛钩-基体（HBB）复合物从球形红杆菌中纯化得到。与沙门氏菌属的HBB相比，该HBB对酸更不稳定，但对热更稳定，并且蛋白质鉴定表明，在此处测试的异养生长条件下，HBB组分仅由球形红杆菌基因组上两组鞭毛基因簇中的一组表达。
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本文引用的文献
1
The home stretch, a first analysis of the nearly completed genome of Rhodobacter sphaeroides 2.4.1.
Photosynth Res. 2001;70(1):19-41. doi: 10.1023/A:1013831823701.
2
The hook gene (flgE) is expressed from the flgBCDEF operon in Rhodobacter sphaeroides: study of an flgE mutant.
J Bacteriol. 2001 Mar;183(5):1680-7. doi: 10.1128/JB.183.5.1680-1687.2001.
3
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J Bacteriol. 2000 Sep;182(18):5218-24. doi: 10.1128/JB.182.18.5218-5224.2000.
4
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J Bacteriol. 1997 Nov;179(21):6581-8. doi: 10.1128/jb.179.21.6581-6588.1997.
5
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6
Purification and characterization of the flagellar hook-basal body complex of Bacillus subtilis.
Mol Microbiol. 1997 Apr;24(2):399-410. doi: 10.1046/j.1365-2958.1997.3341714.x.
7
Physiological and biochemical analyses of FlgH, a lipoprotein forming the outer membrane L ring of the flagellar basal body of Salmonella typhimurium.
J Bacteriol. 1996 Jul;178(14):4200-7. doi: 10.1128/jb.178.14.4200-4207.1996.
8
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
9
Unidirectional, intermittent rotation of the flagellum of Rhodobacter sphaeroides.
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10
Purification and characterization of the flagellar hook-basal body complex of Salmonella typhimurium.
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