L'Hermite A, Lefranc G, Pradal G, André M J, Tusques J
C R Seances Soc Biol Fil. 1975;169(4):1034-40.
In order to attempt identification in vitro of parameters involved in the cellular differenciation, it was necessary to possess a standard material with identical content and distribution of serotonin cells; these two properties are investigated in this paper. Falck's technic is the only sensitive and specific method in demonstrating serotonin young cells devoid of argentaffinity. From 21, 22, 23 and 25 days old rabbit foetuses, 10 mm length duodenal pieces were treated according to Falck and sections serially cut at 8 microns. Some of these specimens were divided prior to Falck treatment in two pieces 3 mm away from the pylorus and in each piece 200 paraffin sections were cut at 8 microns beginning by adjacent ends and progressing in opposite direction. The number of EC sections in 400 whole transverse sections were determined for each foetus. Great variations were observed from one foetus to another, along the intestine, with weight and age. The Student-Fisher test applied to two consecutive pieces of the same duodenum gathering foetuses in groups of 5 animals did not show significative differences from a group to another. Thus, this material can be employed for comparisons under experimental conditions.
为了尝试在体外鉴定参与细胞分化的参数，有必要拥有一种血清素细胞含量和分布相同的标准材料；本文对这两个特性进行了研究。福尔克技术是唯一一种灵敏且特异的方法，用于显示缺乏嗜银性的血清素幼细胞。从21、22、23和25日龄的兔胎儿中，取10毫米长的十二指肠段，按照福尔克方法进行处理，并将其切成8微米厚的连续切片。其中一些标本在福尔克处理前，从幽门处3毫米处分成两片，每片从相邻端开始，以相反方向切成200张8微米厚的石蜡切片。对每个胎儿的400个完整横切片中的肠嗜铬细胞（EC）切片数量进行了测定。在不同胎儿之间、沿着肠道以及随着体重和年龄，观察到了很大的差异。将5只动物一组的胎儿十二指肠的连续两片进行的学生 - 费舍尔检验表明，各组之间没有显著差异。因此，这种材料可用于实验条件下的比较。
