Zheng Jack, Shamsi Shahab A
Department of Chemistry, Center of Biotechnology and Drug Design, Georgia State University, Atlanta, GA 30303, USA.
J Chromatogr A. 2003 Jul 11;1005(1-2):177-87. doi: 10.1016/s0021-9673(03)00886-0.
The capillary electrochromatographic separations of three acidic enantiomers (carprofen, coumachlor and warfarin) were studied on a capillary column packed with 5 microm (3R,4S)-Whelk-O 1 chiral stationary phase. The influence of several experimental parameters (mobile phase pH, type of background electrolyte, acetonitrile ratio, temperature, applied voltage and ionic strength) on electroosmotic flow velocity, retention factor, selectivity factor, efficiency, resolution and effectiveness of chiral separation was evaluated. It was notable that the optimum resolution of the acidic enantiomers was achieved at pH 3.0 phosphate buffer, suggesting that capillary electrochromatography in the ion-suppressed mode can be applied for chiral separations of a range of acidic compounds.
在填充有5微米(3R,4S)-Whelk-O 1手性固定相的毛细管柱上研究了三种酸性对映体（卡洛芬、香豆氯和华法林）的毛细管电色谱分离。评估了几个实验参数（流动相pH值、背景电解质类型、乙腈比例、温度、施加电压和离子强度）对电渗流速度、保留因子、选择性因子、效率、分辨率和手性分离效果的影响。值得注意的是，在pH 3.0的磷酸盐缓冲液中实现了酸性对映体的最佳分离度，这表明离子抑制模式下的毛细管电色谱可用于一系列酸性化合物的手性分离。
