López de Torre B, Tovar J A, Uriarte S, Aldazabal P
Universidad del País Vasco, Facultad de Medicina y Odontología, Servicio de Cirugía Pediátrica.
Cir Pediatr. 1992 Oct;5(4):191-6.
The ever increasing possibilities of prenatal diagnosis and intra-uterine treatment of malformations has created the need for simple and reproducible experimental models on which to build a better knowledge of the biology of the abnormal fetus. Using our own model of experimental gastroschisis (GX) in the chick embryo, we have undertaken to study whether transperitoneal exchanges of water and solutes take place after prolonged visceral exposure to the mixture of amniotic and allantoic fluids which has a greater content in Potassium, Urea, and Proteins and less sodium, chloride and glucose than fetal blood. After surgical evisceration of bowel on the 14th day, incubation was resumed until the 19th day and the embryos were then recovered for weighing and for blood sampling biochemical analysis. The chicks with GX had significantly increased serum Potassium and minimally increased Urea whereas Sodium and Chloride were moderately decreased. Our data confirm that a real peritoneal dialysis with transperitoneal exchange of water and solutes between extraembryonal fluids and the fetal internal environment does indeed take place in this model.
产前诊断和胎儿畸形宫内治疗的可能性不断增加，这就需要建立简单且可重复的实验模型，以便更好地了解异常胎儿的生物学特性。利用我们自己在鸡胚中建立的实验性腹裂（GX）模型，我们研究了在内脏长时间暴露于羊水和尿囊液混合物后，水和溶质是否会发生经腹膜交换。该混合物中钾、尿素和蛋白质的含量高于胎儿血液，而钠、氯和葡萄糖的含量低于胎儿血液。在第14天进行肠道手术去内脏后，继续孵化至第19天，然后取出胚胎进行称重和血液采样生化分析。患有GX的雏鸡血清钾显著升高，尿素略有升高，而钠和氯则适度降低。我们的数据证实，在这个模型中确实发生了胚外液与胎儿内部环境之间经腹膜交换水和溶质的真正腹膜透析。
