嗜热栖热菌HB8伴侣蛋白GroEL-GroES复合物的结晶
Crystallization of the chaperonin GroEL-GroES complex from Thermus thermophilus HB8.
作者信息
Shimamura Tatsuro, Koike-Takeshita Ayumi, Yokoyama Ken, Yoshida Masasuke, Taguchi Hideki, Iwata So
机构信息
Department of Biological Sciences, Imperial College, London SW7 2AZ, England.
出版信息
Acta Crystallogr D Biol Crystallogr. 2003 Sep;59(Pt 9):1632-4. doi: 10.1107/s0907444903013660. Epub 2003 Aug 19.
The chaperonin GroEL-GroES (GroEL/ES) complex from a thermophilic eubacteria, Thermus thermophilus HB8, has been purified and crystallized. The GroEL/ES complex is known to be composed of 14 identical GroEL subunits (58 kDa) and seven identical GroES subunits (11 kDa). The GroEL/ES complex crystals belong to the triclinic space group P1, with unit-cell parameters a = 140.4, b = 156.4, c = 273.1 A, alpha = 82.9, beta = 85.4, gamma = 68.5 degrees. The crystal asymmetric unit contains two molecules (MW = 885 kDa). One data set to 3.0 A resolution, with 383 652 independent observations (89.3% complete) and an R(merge) of 0.08, has been collected from a single crystal. A molecular-replacement solution was obtained using the structure of the GroEL/ES complex from Escherichia coli as a search model.
来自嗜热真细菌嗜热栖热菌HB8的伴侣蛋白GroEL-GroES（GroEL/ES）复合物已被纯化并结晶。已知GroEL/ES复合物由14个相同的GroEL亚基（58 kDa）和7个相同的GroES亚基（11 kDa）组成。GroEL/ES复合物晶体属于三斜晶系空间群P1，晶胞参数为a = 140.4，b = 156.4，c = 273.1 Å，α = 82.9，β = 85.4，γ = 68.5°。晶体不对称单元包含两个分子（分子量 = 885 kDa）。已从单晶收集到一个分辨率为3.0 Å的数据集，有383652个独立观测值（完整性为89.3%），R(merge)为0.08。使用大肠杆菌的GroEL/ES复合物结构作为搜索模型获得了分子置换解。
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