Gyorik Márta, Herpai Zoltán, Szécsényi Ildikó, Varga László, Szigeti Jeno
Institute of Food Science, Faculty of Agricultural and Food Sciences, University of West Hungary, 15-17 Lucsony Street, 9200 Mosonmagyaróvár, Hungary.
J Agric Food Chem. 2003 Aug 27;51(18):5222-5. doi: 10.1021/jf0343635.
The objective of this research was to develop a novel high-performance liquid chromatographic (HPLC) method involving a simple sample preparation procedure for the rapid, low-cost, and sensitive quantitation of phenol in honey at levels of regulatory and practical importance. After proper dilution of honey with water, the samples were analyzed by a gradient HPLC system, using a reversed-phase column with fluorescence detection at excitation and emission wavelengths of 270 and 300 nm, respectively. The eluents applied were water-acetonitrile-85% orthophosphoric acid (10:10:0.01, v/v/v) and water-85% orthophosphoric acid (20:0.01, v/v). The retention time of phenol was found to be 14.1 min, and the limit of quantitation for phenol in honey was set at 5 microg/kg. Overall recovery was 98%. The proposed method has been successfully applied to real sample analysis.
本研究的目的是开发一种新型高效液相色谱（HPLC）方法，该方法具有简单的样品制备程序，可对蜂蜜中具有监管和实际重要性水平的苯酚进行快速、低成本且灵敏的定量分析。用水对蜂蜜进行适当稀释后，使用梯度HPLC系统对样品进行分析，采用反相柱，分别在激发波长和发射波长为270和300 nm处进行荧光检测。所用洗脱液为水 - 乙腈 - 85%正磷酸（10:10:0.01，v/v/v）和水 - 85%正磷酸（20:0.01，v/v）。发现苯酚的保留时间为14.1分钟，蜂蜜中苯酚的定量限设定为5微克/千克。总回收率为98%。所提出的方法已成功应用于实际样品分析。
