高效液相色谱-荧光检测法快速灵敏测定蜂蜜中的苯酚

Rapid and sensitive determination of phenol in honey by high-performance liquid chromatography with fluorescence detection.

作者信息

Gyorik Márta, Herpai Zoltán, Szécsényi Ildikó, Varga László, Szigeti Jeno

机构信息

Institute of Food Science, Faculty of Agricultural and Food Sciences, University of West Hungary, 15-17 Lucsony Street, 9200 Mosonmagyaróvár, Hungary.

出版信息

J Agric Food Chem. 2003 Aug 27;51(18):5222-5. doi: 10.1021/jf0343635.

DOI:10.1021/jf0343635

PMID:12926862

Abstract

The objective of this research was to develop a novel high-performance liquid chromatographic (HPLC) method involving a simple sample preparation procedure for the rapid, low-cost, and sensitive quantitation of phenol in honey at levels of regulatory and practical importance. After proper dilution of honey with water, the samples were analyzed by a gradient HPLC system, using a reversed-phase column with fluorescence detection at excitation and emission wavelengths of 270 and 300 nm, respectively. The eluents applied were water-acetonitrile-85% orthophosphoric acid (10:10:0.01, v/v/v) and water-85% orthophosphoric acid (20:0.01, v/v). The retention time of phenol was found to be 14.1 min, and the limit of quantitation for phenol in honey was set at 5 microg/kg. Overall recovery was 98%. The proposed method has been successfully applied to real sample analysis.

摘要

本研究的目的是开发一种新型高效液相色谱（HPLC）方法，该方法具有简单的样品制备程序，可对蜂蜜中具有监管和实际重要性水平的苯酚进行快速、低成本且灵敏的定量分析。用水对蜂蜜进行适当稀释后，使用梯度HPLC系统对样品进行分析，采用反相柱，分别在激发波长和发射波长为270和300 nm处进行荧光检测。所用洗脱液为水 - 乙腈 - 85%正磷酸（10:10:0.01，v/v/v）和水 - 85%正磷酸（20:0.01，v/v）。发现苯酚的保留时间为14.1分钟，蜂蜜中苯酚的定量限设定为5微克/千克。总回收率为98%。所提出的方法已成功应用于实际样品分析。