Ceballos Laura, Camejo Defrén G, Victoria Fuentes M, Osorio Raquel, Toledano Manuel, Carvalho Ricardo M, Pashley David H
Department of Dental Mater, School of Dentistry, University of Granada, Granada, Spain.
J Dent. 2003 Sep;31(7):469-77. doi: 10.1016/s0300-5712(03)00088-5.
To evaluate the microtensile bond strength of total-etch or self-etch adhesives to caries-affected versus normal dentine, and to correlate these bond strengths with DIAGNOdent laser fluorescence and Knoop microhardness (KH) measurements of the substrates.
Extracted carious human molars were ground to expose flat surfaces where the caries lesion was surrounded by normal dentine. Surfaces were bonded with either Prime & Bond NT, Scotchbond 1, Clearfil SE Bond or Prompt L-Pop, according to manufacturers' recommendations. A crown was built up using resin composite (Tetric Ceram). After storage in water (37 degrees C, 24 h), teeth were vertically serially sectioned into 0.7 mm thick slabs and trimmed to yield 1 mm(2) test area that contained either caries-affected or normal dentine. Samples were tested in tension in an Instron machine at 1 mm/min. The quality of the dentine just beneath each fractured specimen was measured by laser fluorescence and KH.
Total-etch adhesives yielded higher bond strengths than self-etching systems. Significantly lower results were obtained with Prompt L-Pop. All the adhesives attained higher strengths in normal than in caries-affected dentine, but the differences were only significant for Prime & Bond NT and Clearfil SE Bond. Higher laser fluorescence values and lower KH (p<0.001) were recorded in caries-affected dentine compared to normal dentine.
The total-etch adhesives evaluated produced higher bond strengths to normal and caries-affected dentine than self-etching systems. Laser fluorescence measurements discriminated caries-affected dentine from normal dentine, and were strongly correlated with KH. However, laser fluorescence and KH did not permit high correlations with resin-dentine bond strengths in caries-affected dentine.
评估全酸蚀或自酸蚀黏结剂与龋损牙本质及正常牙本质之间的微拉伸黏结强度，并将这些黏结强度与底物的DIAGNOdent激光荧光和努氏显微硬度（KH）测量值进行关联。
将拔除的患龋人磨牙磨平，使龋损病变被正常牙本质包围。根据制造商的建议，使用Prime & Bond NT、Scotchbond 1、Clearfil SE Bond或Prompt L-Pop对表面进行黏结。用树脂复合材料（Tetric Ceram）制作全冠。在水中（37℃，24小时）储存后，将牙齿垂直连续切成0.7毫米厚的薄片，并修整成包含龋损牙本质或正常牙本质的1平方毫米测试区域。样品在Instron机器中以1毫米/分钟的速度进行拉伸测试。通过激光荧光和KH测量每个断裂试样下方牙本质的质量。
全酸蚀黏结剂产生的黏结强度高于自酸蚀系统。Prompt L-Pop的结果明显较低。所有黏结剂在正常牙本质中获得的强度均高于龋损牙本质，但差异仅在Prime & Bond NT和Clearfil SE Bond中显著。与正常牙本质相比，龋损牙本质的激光荧光值更高，KH更低（p<0.001）。
所评估的全酸蚀黏结剂对正常和龋损牙本质产生的黏结强度高于自酸蚀系统。激光荧光测量可区分龋损牙本质和正常牙本质，且与KH密切相关。然而，激光荧光和KH与龋损牙本质中树脂-牙本质黏结强度的相关性不高。
