Yee Karen W L, Moore Susan J, Midmer Michael, Zanke Brent W, Tong Frances, Hedley David, Minden Mark D
Department of Medical Biophysics, University of Toronto, Ontario Cancer Institute, Princess Margaret Hospital, University Health Network, 610 University Avenue, Toronto, Ont., Canada M5G 2M9.
Biochem Biophys Res Commun. 2003 Sep 5;308(4):784-92. doi: 10.1016/s0006-291x(03)01475-x.
We report the cloning of the NKIAMRE gene located on human chromosome 5q31.1. It encodes a novel 52kDa Cdc2-related kinase with a 1.5kb open reading frame. Like MAP kinases, NKIAMRE contains a Thr-X-Tyr (TXY) motif in the activation loop domain. Similar to cdks, NKIAMRE contains the putative negative regulatory Ser14 and Tyr15 residues and the cyclin-binding motif, NKIAMRE, from which it derives its name. Human NKIAMRE has significant amino acid identity to related kinases in rat, mouse, Caenorhabditis elegans, and Drosophila, and is widely expressed in human tissues and cell lines. Confocal microscopy demonstrates that NKIAMRE localizes to the cytoplasm. NKIAMRE is activated by treatment of cells with phorbol 12-myristate 13-acetate. Mutation of the ATP-binding Lys-33 to arginine and the Thr-Glu-Tyr motif to Ala-Glu-Phe abolished its ability to phosphorylate myelin basic protein. NKIAMRE is a member of a conserved family of kinases with homology to both MAP kinases and cyclin-dependent kinases.
我们报道了位于人类5号染色体q31.1上的NKIAMRE基因的克隆。它编码一种具有1.5kb开放阅读框的新型52kDa Cdc2相关激酶。与丝裂原活化蛋白激酶（MAP激酶）一样，NKIAMRE在激活环结构域中含有一个苏氨酸- X -酪氨酸（TXY）基序。与细胞周期蛋白依赖性激酶（cdks）相似，NKIAMRE含有推定的负调控性丝氨酸14和酪氨酸15残基以及细胞周期蛋白结合基序，NKIAMRE由此得名。人类NKIAMRE与大鼠、小鼠、秀丽隐杆线虫和果蝇中的相关激酶具有显著的氨基酸同一性，并且在人类组织和细胞系中广泛表达。共聚焦显微镜显示NKIAMRE定位于细胞质。用佛波酯12 -肉豆蔻酸酯13 -乙酸盐处理细胞可激活NKIAMRE。将ATP结合赖氨酸- 33突变为精氨酸以及将苏氨酸-谷氨酸-酪氨酸基序突变为丙氨酸-谷氨酸-苯丙氨酸会消除其磷酸化髓鞘碱性蛋白的能力。NKIAMRE是一个保守的激酶家族的成员，与MAP激酶和细胞周期蛋白依赖性激酶均具有同源性。
