Iwamuro Shawichi, Sakakibara Michiaki, Terao Megumi, Ozawa Akiko, Kurobe Chizuko, Shigeura Tomokuni, Kato Mayuko, Kikuyama Sakae
Department of Biology, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.
Gen Comp Endocrinol. 2003 Sep;133(2):189-98. doi: 10.1016/s0016-6480(03)00188-6.
Effects of bisphenol A (BPA) on embryonic and larval development were investigated. In Xenopus laevis blastulae treated with 2.5-3.0 x 10(-5) M BPA or with 10(-5) M 17beta estradiol (E2), malformation of the head region, scoliosis (curved vertebrate), and suppression of organogenesis were observed. In addition, 10(-5)-10(-4) M BPA blocked tri-iodothyronine (T3)-inducible resorption of the tail segments from premetamorphic (stage 52-54) larvae in vitro. When stage 52 tadpoles were immersed in 1.0-2.5 x 10(-5) M BPA, deceleration of both spontaneous and thyroxin (T4)-induced metamorphic changes occurred. Furthermore, BPA suppressed thyroid hormone receptor (TR) beta gene expression both in vivo and in vitro. Thus, we concluded that BPA at the concentrations examined affects both embryonic development and larval metamorphosis.
研究了双酚A（BPA）对胚胎和幼体发育的影响。在用2.5 - 3.0×10⁻⁵ M BPA或10⁻⁵ M 17β-雌二醇（E2）处理的非洲爪蟾囊胚中，观察到头部区域畸形、脊柱侧弯（脊椎弯曲）和器官发生受抑制。此外，10⁻⁵ - 10⁻⁴ M BPA在体外阻断了来自前变态期（52 - 54期）幼体的三碘甲状腺原氨酸（T3）诱导的尾段吸收。当52期蝌蚪浸入1.0 - 2.5×10⁻⁵ M BPA中时，自发和甲状腺素（T4）诱导的变态变化均发生减速。此外，BPA在体内和体外均抑制甲状腺激素受体（TR）β基因表达。因此，我们得出结论，在所检测的浓度下，BPA会影响胚胎发育和幼体变态。
