Buell C Robin, Joardar Vinita, Lindeberg Magdalen, Selengut Jeremy, Paulsen Ian T, Gwinn Michelle L, Dodson Robert J, Deboy Robert T, Durkin A Scott, Kolonay James F, Madupu Ramana, Daugherty Sean, Brinkac Lauren, Beanan Maureen J, Haft Daniel H, Nelson William C, Davidsen Tanja, Zafar Nikhat, Zhou Liwei, Liu Jia, Yuan Qiaoping, Khouri Hoda, Fedorova Nadia, Tran Bao, Russell Daniel, Berry Kristi, Utterback Teresa, Van Aken Susan E, Feldblyum Tamara V, D'Ascenzo Mark, Deng Wen-Ling, Ramos Adela R, Alfano James R, Cartinhour Samuel, Chatterjee Arun K, Delaney Terrence P, Lazarowitz Sondra G, Martin Gregory B, Schneider David J, Tang Xiaoyan, Bender Carol L, White Owen, Fraser Claire M, Collmer Alan
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
Proc Natl Acad Sci U S A. 2003 Sep 2;100(18):10181-6. doi: 10.1073/pnas.1731982100. Epub 2003 Aug 19.
We report the complete genome sequence of the model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on tomato and Arabidopsis thaliana. The DC3000 genome (6.5 megabases) contains a circular chromosome and two plasmids, which collectively encode 5,763 ORFs. We identified 298 established and putative virulence genes, including several clusters of genes encoding 31 confirmed and 19 predicted type III secretion system effector proteins. Many of the virulence genes were members of paralogous families and also were proximal to mobile elements, which collectively comprise 7% of the DC3000 genome. The bacterium possesses a large repertoire of transporters for the acquisition of nutrients, particularly sugars, as well as genes implicated in attachment to plant surfaces. Over 12% of the genes are dedicated to regulation, which may reflect the need for rapid adaptation to the diverse environments encountered during epiphytic growth and pathogenesis. Comparative analyses confirmed a high degree of similarity with two sequenced pseudomonads, Pseudomonas putida and Pseudomonas aeruginosa, yet revealed 1,159 genes unique to DC3000, of which 811 lack a known function.
我们报道了模式细菌病原体丁香假单胞菌番茄致病变种DC3000（DC3000）的全基因组序列，该菌株可侵染番茄和拟南芥。DC3000基因组（650万个碱基对）包含一条环状染色体和两个质粒，共编码5763个开放阅读框（ORF）。我们鉴定出298个已确定的和推测的毒力基因，包括几个基因簇，这些基因簇编码31个已确认的和19个预测的III型分泌系统效应蛋白。许多毒力基因是旁系同源家族的成员，并且也靠近移动元件，这些移动元件总共占DC3000基因组的7%。该细菌拥有大量用于获取营养物质（特别是糖类）的转运蛋白，以及与附着在植物表面相关的基因。超过12%的基因专门用于调控，这可能反映了在附生生长和致病过程中快速适应不同环境的需求。比较分析证实，DC3000与另外两种已测序的假单胞菌——恶臭假单胞菌和铜绿假单胞菌高度相似，但也揭示了DC3000特有的1159个基因，其中811个基因的功能未知。
