Vinck Elke M, Cagnie Barbara J, Cornelissen Maria J, Declercq Heidi A, Cambier Dirk C
Department of Rehabilitation Sciences and Physiotherapy, Ghent University, 9000 Ghent, Belgium.
Lasers Med Sci. 2003;18(2):95-9. doi: 10.1007/s10103-003-0262-x.
As Light Emitting Diode (LED) devices are commercially introduced as an alternative for Low Level Laser (LLL) Therapy, the ability of LED in influencing wound healing processes at cellular level was examined.
STUDY DESIGN/MATERIALS AND METHODS: Cultured fibroblasts were treated in a controlled, randomized manner, during three consecutive days, either with an infrared LLL or with a LED light source emitting several wavelengths (950 nm, 660 nm and 570 nm) and respective power outputs. Treatment duration varied in relation to varying surface energy densities (radiant exposures).
Statistical analysis revealed a higher rate of proliferation (p < 0.001) in all irradiated cultures in comparison with the controls. Green light yielded a significantly higher number of cells, than red (p < 0.001) and infrared LED light (p < 0.001) and than the cultures irradiated with the LLL (p < 0.001); the red probe provided a higher increase (p < 0.001) than the infrared LED probe and than the LLL source.
LED and LLL irradiation resulted in an increased fibroblast proliferation in vitro. This study therefore postulates possible stimulatory effects on wound healing in vivo at the applied dosimetric parameters.
随着发光二极管（LED）设备作为低强度激光（LLL）治疗的替代品被商业引入，研究了LED在细胞水平影响伤口愈合过程的能力。
研究设计/材料与方法：培养的成纤维细胞以受控、随机的方式连续三天接受治疗，分别使用红外低强度激光或发射多种波长（950纳米、660纳米和570纳米）及相应功率输出的LED光源。治疗持续时间根据不同的表面能量密度（辐射暴露量）而变化。
统计分析显示，与对照组相比，所有受辐照培养物中的增殖率更高（p < 0.001）。绿光产生的细胞数量显著多于红光（p < 0.001）、红外LED光（p < 0.001）以及接受低强度激光辐照的培养物（p < 0.001）；红色探头产生的细胞增加量高于红外LED探头和低强度激光光源（p < 0.001）。
LED和低强度激光辐照导致体外成纤维细胞增殖增加。因此，本研究推测在所应用的剂量参数下，对体内伤口愈合可能具有刺激作用。
