Imai Takashi, Fujita Tetsuji, Yamazaki Yoji
Department of Surgery, Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan.
Surg Today. 2003;33(9):684-7. doi: 10.1007/s00595-003-2585-4.
Although many studies have shown the beneficial effects of lipoproteins on animals with endotoxemia, little is known about the impact of apolipoprotein A-I (apoA-I) on tumor necrosis factor Alpha (TNF-Alpha) release in response to lipopolysaccharide (LPS). The present study was conducted to determine whether the administration of apoA-I inhibits the release of TNF-Alpha and influences the survival rate of rats with endotoxemia.
Forty male Wistar rats were divided randomly into four groups. Rats in the first and second groups were given 1 mg/kg LPS intraperitoneally (i.p.) and blood was collected 1 h later to measure the serum levels of TNF-Alpha. Either 10 mg/kg apoA-I or Tris-buffered saline was injected i.p. and the serum TNF-Alpha levels were measured again 2 h later. Rats in the third and fourth groups were given 5 mg/kg LPS. Following the administration of 10 mg/kg apoA-I or Tris-buffered saline, animals were observed for 5 days and survival rates were determined.
ApoA-I inhibited the release of serum TNF-Alpha and improved the survival rates of rats with endotoxemia.
The administration of apoA-I suppressed the TNF-Alpha release in endotoxemia and decreased the mortality rates of rats.
