Ivanova Anna, Nakahira Eiko, Kagawa Tetsushi, Oba Akio, Wada Tamaki, Takebayashi Hirohide, Spassky Nathalie, Levine Joel, Zalc Bernard, Ikenaka Kazuhiro
Laboratory of Neural Information, National Institute for Physiological Sciences, Okazaki National Research Institutes, Aichi, Japan.
J Neurosci Res. 2003 Sep 1;73(5):581-92. doi: 10.1002/jnr.10717.
The existing view is that cortical oligodendrocytes (OLs) in rodents are born from the cortical subventricular zone (SVZ) after birth, but recent data suggest that many forebrain oligodendrocyte progenitor cells (OPCs) are specified much earlier (between E9.5 and E13.5 in the mouse) in the ventricular zone of the ventral forebrain under the control of sonic hedgehog (Shh) and migrate into the cortex afterward. We examined expression of specific early OL markers (PDGFRalpha, PLP/DM20, Olig2, and NG2) in the developing forebrain to clarify this issue. We propose that OPCs colonize the developing cortex in two temporally distinct waves. The gray matter is at least partially populated by a first wave of OPCs that arises in the medial ganglionic eminence and the entopeduncular area and spreads into the cortex via the developing cortical plate. The cerebral cortex benefits from the second wave of OPCs coming from residential SVZ. In the second wave, there might be two different types of precursor cells: PLP/DM20(+) cells populating only inner layers and PDGFRalpha(+) cells, which might eventually myelinate the outer regions as well.
目前的观点认为，啮齿动物的皮质少突胶质细胞（OLs）在出生后源自皮质室下区（SVZ），但最近的数据表明，许多前脑少突胶质细胞祖细胞（OPCs）在 Sonic hedgehog（Shh）的控制下，在腹侧前脑的脑室区更早（小鼠胚胎期第9.5天至13.5天之间）就已特化，随后迁移至皮质。我们检测了发育中的前脑中特定早期OL标志物（血小板衍生生长因子受体α（PDGFRα）、髓鞘蛋白脂蛋白/二甲基精氨酸蛋白20（PLP/DM20）、少突胶质细胞转录因子2（Olig2）和神经胶质抗原2（NG2））的表达，以阐明这一问题。我们提出，OPCs以两个时间上不同的波峰定殖于发育中的皮质。灰质至少部分由源自内侧神经节隆起和内囊前区的第一波OPCs填充，并通过发育中的皮质板扩散至皮质。大脑皮质受益于来自室管膜下区固有细胞群的第二波OPCs。在第二波中，可能存在两种不同类型的前体细胞：仅填充内层的PLP/DM20(+)细胞和可能最终也使外层髓鞘化的PDGFRα(+)细胞。
