Kimkong Ingorn, Mutirangura Apiwat, Pimtanothai Nattiya
Inter-Department of Medical Microbiology, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand.
J Med Assoc Thai. 2003 Jun;86 Suppl 2:S230-6.
The aim of this study was to investigate the distribution of human leukocyte antigens (HLA) -E alleles in Thailand. HLA-E alleles were assigned by using a polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) method and direct sequencing in 200 healthy individuals. They comprised 100 Thai, 50 Chinese and 50 Thai-Chinese. From the results, three alleles of HLA-E could be detected in these populations. The E0101 was the most common allele in Thai and Thai-Chinese with allelic frequencies of 42.5 per cent and 38 per cent, respectively. The other HLA-E allele frequencies of Thai origin were 33 per cent for E01031 and 24.5 per cent for E01032, respectively. Among Thai-Chinese, the allele frequencies of HLA-E were 31 per cent for E01031 and E01032, respectively. Whereas, the E01031 was the predominant allele in Chinese origin with a frequency of 39 per cent, followed by E0101 and E01032 with 32 per cent and 29 per cent, respectively. No E01033, E0102 and E*0104 could be detected in all individuals. When comparing the distribution of HLA-E alleles between each of the populations (Thai vs Chinese, Thai vs Thai-Chinese and Chinese vs Thai-Chinese), no significant difference could be found among these populations. In addition, there was no significant difference of the distribution of HLA-E alleles between the study populations and other populations from Asian countries, reported previously. However, there were significant differences between the populations (Thai, Chinese and Thai-Chinese) and Danish (chi2 = 15.64, p = 0.0004; chi2 = 24.58, p = 0.0000046; chi2 = 14.69, p = 0.00065, respectively).
本研究旨在调查泰国人群中人类白细胞抗原（HLA）-E等位基因的分布情况。采用聚合酶链反应-序列特异性寡核苷酸探针（PCR-SSOP）方法和直接测序法，对200名健康个体的HLA-E等位基因进行分型。这些个体包括100名泰国人、50名中国人和50名泰籍华人。结果显示，在这些人群中可检测到3种HLA-E等位基因。E0101是泰国人和泰籍华人中最常见的等位基因，其等位基因频率分别为42.5%和38%。泰国人群中其他HLA-E等位基因频率分别为：E01031为33%，E01032为24.5%。在泰籍华人中，E01031和E01032的等位基因频率分别为31%。而在中国人中，E01031是主要等位基因，频率为39%，其次是E0101和E01032，频率分别为32%和29%。在所有个体中均未检测到E01033、E0102和E*0104。比较各人群（泰国人与中国人、泰国人与泰籍华人、中国人与泰籍华人）之间HLA-E等位基因的分布情况，发现这些人群之间无显著差异。此外，本研究人群与先前报道的其他亚洲国家人群的HLA-E等位基因分布也无显著差异。然而，泰国人、中国人和泰籍华人这三个人群与丹麦人群之间存在显著差异（卡方值分别为15.64，p = 0.0004；24.58，p = 0.0000046；14.69，p = 0.00065）。
