Banerjee S, Dey N, Majumder G C
Indian Institute of Chemical Biology, Calcutta.
Indian J Exp Biol. 1992 Nov;30(11):1056-61.
The maturing goat epididymal spermatozoa were isolated from different segments of epididymis and these cells dispersed in a modified Ringer's solution, were incubated at 37 degrees C for 60 min to evaluate their autoagglutination efficacy. Distal corpus-epididymal spermatozoa specifically showed high order of head-to-head autoagglutination property whereas all other sperm cells did not show any detectable aggregation. The goat epididymal plasma has been shown to possess an anti-agglutinin that markedly inhibits sperm agglutination phenomenon and also dissociates the cells from the sperm clusters. Epididymal plasma is the most potent source of the anti-agglutinin which is a heat-stable specific glycoprotein. Like the autoagglutination phenomenon, the initiation of sperm forward progression also starts in the distal-corpus epididymis. The temporal correlation of these two events suggests that sperm autoagglutination may be a prerequisite for the induction of flagellar motility during the epididymal maturity of male gametes.
从山羊附睾的不同节段分离出成熟的附睾精子，将这些分散在改良林格氏液中的细胞在37℃孵育60分钟，以评估其自身凝集效果。附睾体远端的精子特别表现出高度的头对头自身凝集特性，而所有其他精子细胞未显示出任何可检测到的聚集。山羊附睾血浆已被证明具有一种抗凝集素，它能显著抑制精子凝集现象，并使细胞从精子团块中解离。附睾血浆是抗凝集素最有效的来源，抗凝集素是一种热稳定的特异性糖蛋白。与自身凝集现象一样，精子向前运动的起始也始于附睾体远端。这两个事件的时间相关性表明，精子自身凝集可能是雄性配子在附睾成熟过程中诱导鞭毛运动的先决条件。
